Park 2010 Sensors and Actuators B: Difference between revisions
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{{Publication | {{Publication | ||
|title=Park J, Pak YMK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sensors and Actuators B 147: | |title=Park J, Pak YMK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sensors and Actuators B 147:263โ9. | ||
|info=[http://www.sciencedirect.com\science\article\pii\S0925400510002959 ScienceDirect] | |info=[http://www.sciencedirect.com\science\article\pii\S0925400510002959 ScienceDirect] | ||
|authors=Park J, Pak YMK, Pak JJ | |authors=Park J, Pak YMK, Pak JJ |
Revision as of 14:36, 4 March 2015
Park J, Pak YMK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sensors and Actuators B 147:263โ9. |
Park J, Pak YMK, Pak JJ (2010) Sensors and Actuators B: Chemical
Abstract: This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate(OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate (OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode (WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The fabricated sensor showed a sensitivity of 2.84 A/cm2M and a 90% response time of 4.9 s in an average of 5 sensors when a 25,000ยตm2 WE and a 20ยตm polydimethylsiloxane membrane were used. This is the first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05ยฑ1.87 nA at the full-oxygen state and 2.77ยฑ1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69ยฑ0.30 was almost identical to the result of 3.70ยฑ0.26 obtained from a commercial system. first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05ยฑ1.87 nA at the full-oxygen state and 2.77ยฑ1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69ยฑ0.30 was almost identical to the result of 3.70ยฑ0.26 obtained from a commercial system. โข Keywords: electrochemistry, reservoir-type, oxygen sensor, cellular respiration, oxygen consumption rate
โข O2k-Network Lab: KR Seoul Pak YMK
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Organism: Rat
Tissue;cell: Skeletal muscle
Preparation: Intact cells
HRR: Oxygraph-2k
L6 rat skeletal muscle