Difference between revisions of "PM-pathway control state"
From Bioblast
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|description=[[File:M.jpg|left|200px|PM]] '''PM''': [[Pyruvate]] & [[Malate]]. | |description=[[File:M.jpg|left|200px|PM]] '''PM''': [[Pyruvate]] & [[Malate]]. | ||
'''MitoPathway control:''' | '''MitoPathway control state:''' N | ||
'''SUIT protocol:''' [[SUIT_FNSGp(PGM)01]] - SUIT_RP1 | '''SUIT protocol:''' [[SUIT_FNSGp(PGM)01]] - SUIT_RP1 | ||
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== | == Linear coupling control in the N-pathway control state: ''L β P - E'' == | ||
::::* '''''L - P''''' | ::::* '''''L - P''''' | ||
:::: [[OXPHOS coupling efficiency]] (''P-L'' or ''βP'' control factor), ''j<sub>βP</sub>'' = ''βP/P'' = (''P-L'')/''P'' = 1-''L/P'', is measured in the CI-linked substrate state, with defined coupling sites (CI, CIII, CIV) and at high flux. | :::: [[OXPHOS coupling efficiency]] (''P-L'' or ''βP'' control factor), ''j<sub>βP</sub>'' = ''βP/P'' = (''P-L'')/''P'' = 1-''L/P'', is measured in the CI-linked substrate state, with defined coupling sites (CI, CIII, CIV) and at high flux. | ||
Revision as of 10:36, 8 November 2016
- high-resolution terminology - matching measurements at high-resolution
PM-pathway control state
Description
MitoPathway control state: N
SUIT protocol: SUIT_FNSGp(PGM)01 - SUIT_RP1
Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). 2-oxoglutarate (Ξ±-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase).
Abbreviation: PM
Reference: Gnaiger 2014 MitoPathways - Chapter 3.2
MitoPedia concepts:
Respiratory state,
SUIT state
PM(L)
- SUIT_FNSGp(PGM)01: 1PM 2D&Dc (&DcNADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd
PM(P)
- SUIT_FNSGp(PGM)01: 1PM 2D&Dc (&DcNADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd
PM(E)
- SUIT_FNSGp(PGM)01: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm
Linear coupling control in the N-pathway control state: L β P - E
- L - P
- OXPHOS coupling efficiency (P-L or βP control factor), jβP = βP/P = (P-L)/P = 1-L/P, is measured in the CI-linked substrate state, with defined coupling sites (CI, CIII, CIV) and at high flux.
- P - E
- CCCP is titrated stepwise to maximum flux, to evaluate limitation of OXPHOS by the phosphorylation system, expressed as the apparent excess E-P capacity factor (E-P coupling control factor), jExP = (E-P)/E = 1-P/E. If jExP>0, then the ETS coupling efficiency rather than the OXPHOS coupling efficiency is the proper expression of coupling, jβE = βE/E = (E-L)/E = 1-L/E.
Discussion
- Pyruvate alone is not an ETS competent substrate state in most mt-preparations, since acetyl-CoA accumulates without the co-substrate (oxaloacetate) of citrate synthase.
- Malate control state (M alone) is not an ETS competent substrate state in many mt-preparations, since oxaloacetate accumulates without the co-substrate (acetyl-CoA) of citrate synthase.
