Difference between revisions of "PM-pathway control state"
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{{MitoPedia | {{MitoPedia | ||
|abbr=PM | |abbr=PM | ||
|description='''PM''': [[Pyruvate]] & [[Malate]]. | |description=[[File:M.jpg|left|200px|PM]] '''PM''': [[Pyruvate]] & [[Malate]]. | ||
'''MitoPathway control:''' CI | '''MitoPathway control:''' CI | ||
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[[File:PM.jpg|right|400px|link=Gnaiger 2014 MitoPathways |Gnaiger 2014 MitoPathways - Chapter 3.2]] | [[File:PM.jpg|right|400px|link=Gnaiger 2014 MitoPathways |Gnaiger 2014 MitoPathways - Chapter 3.2]] | ||
== PM(L) == | == PM(L) == | ||
* [[SUIT-RP1]]: '''<U>1PM</U>''' 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm | ::::* [[SUIT-RP1]]: '''<U>1PM</U>''' 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm | ||
== PM(P) == | == PM(P) == | ||
* [[SUIT-RP1]]: 1PM '''<U>2D</U>''' 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm | ::::* [[SUIT-RP1]]: 1PM '''<U>2D</U>''' 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm | ||
== PM(E) == | == PM(E) == | ||
* [[SUIT-RP1]]: 1PM 2D 2c (2NADH) '''<U>3U</U>''' 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm | ::::* [[SUIT-RP1]]: 1PM 2D 2c (2NADH) '''<U>3U</U>''' 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm | ||
== CI-linked linear coupling control: ''L β P - E'' == | == CI-linked linear coupling control: ''L β P - E'' == | ||
* '''''L - P''''' | ::::* '''''L - P''''' | ||
: [[OXPHOS coupling efficiency]] (''P-L'' or ''βP'' control factor), ''j<sub>βP</sub>'' = ''βP/P'' = (''P-L'')/''P'' = 1-''L/P'', is measured in the CI-linked substrate state, with defined coupling sites (CI, CIII, CIV) and at high flux. | :::: [[OXPHOS coupling efficiency]] (''P-L'' or ''βP'' control factor), ''j<sub>βP</sub>'' = ''βP/P'' = (''P-L'')/''P'' = 1-''L/P'', is measured in the CI-linked substrate state, with defined coupling sites (CI, CIII, CIV) and at high flux. | ||
* '''''P - E''''' | ::::* '''''P - E''''' | ||
: [[CCCP]] is titrated stepwise to maximum flux, to evaluate limitation of OXPHOS by the phosphorylation system, expressed as the apparent [[excess E-P capacity factor |excess ''E-P'' capacity factor]] (''E-P'' coupling control factor), ''j<sub>ExP</sub>'' = (''E-P'')/''E'' = 1-''P/E''. If ''j<sub>ExP</sub>''>0, then the [[ETS coupling efficiency]] rather than the [[OXPHOS coupling efficiency]] is the proper expression of coupling, ''j<sub>βE</sub>'' = ''βE/E'' = (''E-L'')/''E'' = 1-''L/E''. | :::: [[CCCP]] is titrated stepwise to maximum flux, to evaluate limitation of OXPHOS by the phosphorylation system, expressed as the apparent [[excess E-P capacity factor |excess ''E-P'' capacity factor]] (''E-P'' coupling control factor), ''j<sub>ExP</sub>'' = (''E-P'')/''E'' = 1-''P/E''. If ''j<sub>ExP</sub>''>0, then the [[ETS coupling efficiency]] rather than the [[OXPHOS coupling efficiency]] is the proper expression of coupling, ''j<sub>βE</sub>'' = ''βE/E'' = (''E-L'')/''E'' = 1-''L/E''. | ||
== Discussion == | == Discussion == | ||
:::: [[Pyruvate alone]] is not an ETS competent substrate state in most mt-preparations, since acetyl-CoA accumulates without the co-substrate (oxaloacetate) of citrate synthase. | |||
:::: [[Malate alone]] is not an ETS competent substrate state in many mt-preparations, since oxaloacetate accumulates without the co-substrate (acetyl-CoA) of citrate synthase. | |||
Revision as of 18:58, 25 February 2016
- high-resolution terminology - matching measurements at high-resolution
PM-pathway control state
Description
MitoPathway control: CI
SUIT protocol: SUIT-RP1
Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). 2-oxoglutarate (Ξ±-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase).
Abbreviation: PM
Reference: Gnaiger 2014 MitoPathways - Chapter 3.2
MitoPedia O2k and high-resolution respirometry: "SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
SUIT state"SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
PM(L)
- SUIT-RP1: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm
PM(P)
- SUIT-RP1: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm
PM(E)
- SUIT-RP1: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm
CI-linked linear coupling control: L β P - E
- L - P
- OXPHOS coupling efficiency (P-L or βP control factor), jβP = βP/P = (P-L)/P = 1-L/P, is measured in the CI-linked substrate state, with defined coupling sites (CI, CIII, CIV) and at high flux.
- P - E
- CCCP is titrated stepwise to maximum flux, to evaluate limitation of OXPHOS by the phosphorylation system, expressed as the apparent excess E-P capacity factor (E-P coupling control factor), jExP = (E-P)/E = 1-P/E. If jExP>0, then the ETS coupling efficiency rather than the OXPHOS coupling efficiency is the proper expression of coupling, jβE = βE/E = (E-L)/E = 1-L/E.
Discussion
- Pyruvate alone is not an ETS competent substrate state in most mt-preparations, since acetyl-CoA accumulates without the co-substrate (oxaloacetate) of citrate synthase.
- Malate alone is not an ETS competent substrate state in many mt-preparations, since oxaloacetate accumulates without the co-substrate (acetyl-CoA) of citrate synthase.
