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Instrumental background oxygen flux [MiPNet14.06] in a respirometer is due to oxygen consumption by the POS, and oxygen diffusion into or out of the aqueous medium in the O2k-chamber. It is measured in the range of experimental oxygen levels by a standardized instrumental background test, and is a property of the instrumental system. Instrumental background correction eliminates errors by systemic flux compensation, automatically performed by DatLab.
References
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [pdf].
- high-resolution terminology - matching measurements at high-resolution
Oxygen flux - instrumental background