O2k-Workshops in O2k-Network Labs

Concept

OROBOROS O2k-Workshops are organized in cooperation with O2k-Network Labs as a service for the O2k-Network Lab, and feedback support for OROBOROS INSTRUMENTS to disseminate the newest developments in the region by invitation to external participants.

O2k-Workshop programme

• General: Focus on four sessions:

1. Setting up the Oxygraph-2k and instrumental service.
2. Protocols for high-resolution respirometry and O2k-Fluorometry: focus on combined measurement of respiration and mt-membrane potential.
3. O2k-Demo experiment: Combined measurement of respiration and hydrogen peroxide production.
4. Extending real-time data analysis to evaluation of results, discussion of protocol design, OROBOROS in science.

• A detailed programme including the timing of sessions is developed in collaboration with the local host institution.

IOC in O2k-Network Labs

Further IOC in O2k-Network Labs

2013

• DK_Copenhagen_Dela F

2012

• ES_Madrid_Cadenas S

2011

• >>IOC063

2010

• LT_Kaunas_Borutaite V

2008

• >>IOC048

O2k-Workshops are listed as MitoGlobal Events.

O2k-Workshops and expeditions: environments at high altitude and cold

Oxygen delivery to the tissue is a critically limiting factor frequently studied in high altitude environments and uner hypothermia. Very few investigations, however, address the equally important metabolic process of cellular oxygen utilization under short-term and long-term hypobaric hypoxia. OROBOROS INSTRUMENTS is a highly competent potential partner of a multi-disciplinary and international research group focused on clinical and biological aspects of Human life at high altitude. We have participated in several expeditions to high altitude to study short-term acclimatization and adaptation of Human populations to high altitude and low temperature (Monte Rosa, Italy; Mt. Chacaltaya, Bolivia; Everest Base Camp, Nepal; Alaska, USA; Spitsbergen, Norway). We are specialists in instrumental development and applications in mitochondrial physiology and have taken high-resolution respirometry to the extremes of field work at high altitude, with long-term experience in the organization of workshops all-over the world. In methodological and technical workshops, participants are introduced to the conceptual and practical basis of the diagnosis of mitochondrial function and dysfunction. Specifically, the boundary conditions prevailing in laboratories available at high altitude can be taking into account, which require the highest performance standards for electronic instruments and their application. In addition we offer pre-expedition training to international partners in our research laboratory, for scientists and students to gain competence for the challenges met when working in high altitude environments. As a long-term perspective, we aim at elaborating standards for the evaluation of cell respiration and mitochondrial function to diagnose diseases and find potential targets to improve the state of health and quality of Human life in high altitude and hypothermic environments. These standards can be implemented not only in the participating countries but may become a template for world-wide dissemination.

Reference and links

1. Previous expeditions: OROBOROS expeditions
2. International workshops: OROBOROS O2k-Workshops
3. OROBOROS Oxygraph-2k publications on hypoxia and mitochondrial function: O2k-Publications:_Hypoxia

Selected publications on hypoxia and mitochondrial function

1. Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.
2. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
3. Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.
4. Rostrup M, Fossbakk A, Hauge A, Kleppe R, Gnaiger E, Haavik J (2008) Oxygen dependence of tyrosine hydroxylase. Amino Acids 34: 455-464.
5. Aragonés J et al (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180.
6. Sommer N, Pak O, Schörner S, Derfuss T, Krug A, Gnaiger E, Ghofrani HA, Schermuly RT, Huckstorf C, Seeger W, Grimminger F, Weissmann N (2010) Mitochondrial cytochrome redox states and respiration in acute pulmonary oxygen sensing. Eur Respir J 36: 1056-1066.
7. Smolková K, Bellance N, Scandurra F, Génot E, Gnaiger E, Plecitá-Hlavatá L, Ježek P, Rossignol R (2010) Mitochondrial bioenergetic adaptations of breast cancer cells to aglycemia and hypoxia. J Bioenerg Biomembr 42: 55-67.
8. Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.
9. Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
10. Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
11. Jacobs RA, Boushel RC, Wright-Paradis C, Calbet JA, Robach P, Gnaiger E, Lundby C (2013) Mitochondrial function in human skeletal muscle following high altitude exposure. Exp Physiol 98: 245-255.