O2k-Titration Set: Difference between revisions

Latest revision as of 17:46, 11 October 2022

O2k-Titration Set

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm³ and 3 spare plungers, 6 x 25 mm³, 1 x 50 mm³, 1 x 100 mm³, 1 x 500 mm³; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks.

Reference: Product details and purchasing information

Additional resources

»MiPNet09.12 O2k-Titrations

»MiPNet19.14 SOP Hamilton microsyringes

Note: Titration of chemicals in respirometry

Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.

After a titration, it is common to observe liquid accumulated on top of the stopper receptacle. When this happens, we recommend removing this excess of liquid as it can lead to O₂ backdiffusion into the chamber. For this, we recommend using the ISS, placing the tip close to the capillary, but not directly on top of it.

Further information

Safety information: fixed needles are used with rounded tips, not usable for injection into tissues.
Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions.
Visit also: Hamilton.
Eliminate rough edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing.
Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H₂O and try to press the plunger gently down.

MitoPedia O2k and high-resolution respirometry: O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry

@@ Line 1: / Line 1: @@
-{{Mainpage orobopedia}}
+{{Technical support}}
-{{Product
+__TOC__
-|description=The '''Titration Set''' consists of 6x 10 µl, 6x 25 µl, and 2x 50 µl Hamilton glass syringes, and 3 spare plungers for the 10 µl syringes. It is a basic HRR-Accessory included in the [[O2k-Core]] Unit.
+{{MitoPedia without banner
-|product id=20820-01
+|abbr=
-|product type=O2k, O2k-Module, O2k-Core, Microsyringe, Catalogue
+|description=[[Image:O2k-Titration Set.JPG|right|180px]] The '''O2k-Titration Set''' consists of Hamilton microsyringes (6 x 10 mm<sup>3</sup> and 3 spare plungers, 6 x 25 mm<sup>3</sup>, 1 x 50 mm<sup>3</sup>, 1 x 100 mm<sup>3</sup>, 1 x 500 mm<sup>3</sup>; fixed needles with rounded tips), provided in the [[Syringe Storage Box]] with [[Syringe Labels]], a set of two [[Syringe Racks]] with [[Syringe Collars]], and a set of two [[Tube Racks]].
-|info=[[MiPNet09.12 O2k-Titrations]]
-|product image=[[File:Hamilton Syringes for Manual Titration.jpg|right|180px|link=]]
+|info= [https://www.oroboros.at/index.php/product/o2k-titration-set/ '''Product details and purchasing information''']
 }}
-== The '''Titration Set''' ''consists of'' ==
+== Additional resources ==
+:::: »[[MiPNet09.12 O2k-Titrations]] </br>
+:::: »[[MiPNet19.14 SOP Hamilton microsyringes]]
-:6 [[Microsyringe\10 mm3 51/0.13 mm]] 10 mm<sup>3</sup>
-:6 [[Microsyringe\25 mm3 51/0.15 mm]] 25 mm<sup>3</sup>
-:2 [[Microsyringe\50 mm3 51/0.15 mm]] 50 mm<sup>3</sup>
-:3 [[Plunger\10 mm3]] for 10 mm<sup>3</sup> syringe
+== Note: Titration of chemicals in respirometry ==
+:::: Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
-== Handling of the syringes ==
+::::  After a titration, it is common to observe liquid accumulated on top of the stopper receptacle. When this happens, we recommend removing this excess of liquid as it can lead to O<sub>2</sub> backdiffusion into the chamber. For this, we recommend using the [[ISS]], placing the tip close to the capillary, but not directly on top of it.
-* separate inhibitors from substrates
-* rinse the outside with water immediately after use
+=== Further information ===
-* at the start of a day: rinse the outside with the pure solvent and wash the syringes 3 times with the pure solvent
+:::* Safety information: fixed needles are used with rounded tips, not usable for injection into tissues.
-* rinse with pure solvent before use
+:::* Hamilton microsyringes are specifically produced for O2k-titrations and can be ordered directly from Oroboros Instruments to assure exact fitting with the O2k-Stopper and O2k-Chamber dimensions.
-* between two runs during the day: rinse the outside with water
+:::* Visit also: [http://www.hamiltoncompany.com/products/syringes-and-needles Hamilton].
-* at the end of a day: wash 3 x with last used solvent, rinse 3 x with EtOH 100 %
+:::* Eliminate rough edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing.
-* storage: ethanol 100% (short), dry (longtime)
+:::* Use a cleaning wire for the needle. If a syringe is clogged , sometimes it is also helpful to fill it from the back with EtOH or H<sub>2</sub>O and try to press the plunger gently down.
+{{MitoPedia O2k and high-resolution respirometry
+|mitopedia O2k and high-resolution respirometry=O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
+}}