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We provide service and support based on open innovation
O2k-Open Support tips from the Oroboros Science Agenda
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). Further details in MitoPedia: »Oxygen calibration - DatLab
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. Further details in MitoPedia: Oxygen calibration - DatLab
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark ‘R1’ andclick on Calibrate and copy to clipboard in the O2 calibration window. Further details in MitoPedia: Oxygen calibration - DatLab
- Open Innovation in the NextGen-O2k software development DatLab 8. Here is a summary of MitoPedia pages including DatLab 8 perspectives
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. Further details » O2k configuration
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). Further details » O2k-Chamber MitoPedia
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- The O2 solubility factor varies according to medium used and influence the air calibration. Further details » Oxygen solubility factor
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?