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O2k-Feedback

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Revision as of 23:16, 10 June 2014 by Gnaiger Erich (talk | contribs)

                

O2k-Feedback

O2k-Catalogue

Description O2k-Feedback: OROBOROS - Cooperation and Feedback in Science
Product ID
Type O2k, Feedback, Catalogue
Link http://www.oroboros.at/?HRR-Feedback
Image
OROBOROS-solo.png


We thank you for your positive feedback. This is the energy that fuels our developments and encourages us to strive towards further optimization and innovation, along the lines and circles of the Oroboros, in the spirit of Gentle Science. Thank you for your collaboration! - The OROBOROS-Team


O2k-Feedback


O2k-feedback 2014

  • We are more than happy with the O2k. In fact, it is one of the most useful instruments we have in both our laboratories. Jirka Neuzil (2014-06-10).
  • I have been a proud O2k user for the last year and a half, and I and my colleagues have gotten so much use out of our first instrument that we would like to explore the purchase of a second system. Thank you so much for your kind assistance! - Joshua Patrick Fessel, Vanderbilt University School of Medicine, Department of Medicine, Division of Allergy, Pulmonary and Critical Care Medicine, TN, US.
  • Thank you for the "Blue book"! I was surprised when I found the conference 2013 book with the Blue book [...] and I felt disappointed because I couldn't attend the conference... This book is full of interesting topics, lectures and abstracts! - Igor Golic, University of Belgrade, Cell and Tissue Biology Dept., RS.


O2k-feedback 2013

  • Thanks to you and the entire team for your outstanding assistance this year, and moreso for your pleasant friendliness. - Christopher G Perry, York University, School of Kinesiology and Health Science, CA.
  • Thank you very much. I have never worked with a company with this fantastic information system. - Morales-Alamo D.
  • 'High resolution designs (i.e., O2k, OROBOROS Instruments) maximize respirometric sensitivity and precision (minimal O2 leak and highly sensitive electrodes), reducing the biological sample size required. Software advances in flux derivations of changes in chamber PO2 also permit real-time reporting of respiratory kinetics (Datlab, OROBOROS Instruments), which improves data analyses over other systems requiring visual assessments of steady-state kinetics.' - Christopher G Perry, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.
  • Thanks again for being so quick with assessing it, repairing and returning the Oxygraph. Working with Oroboros is so much more superior to working with other companies. - Lauren M Sparks, Translations Research Institute for Metabolism and Diabetes, Orlando, FL, US.
  • Everything what concerns OROBOROS company (oxygraphs, company staff, workshops, conferences, scientific discussions, the quality of information, etc.) works at the highest level !!!!!!! BRAVO for you :) - Magdalena Labieniec-Watala, University of Lodz, Poland.
  • In initial experiments with the Oxygraph we noticed improved sensitivity and stability compared to another polarographic system used by us for many years. - Brian Fink, VA Medical Center, Iowa, US.


O2k-Feedback 2012

  • I'm very impressed and love the logo. - I just have to say it … again, I love your logo. - Anna Gibson, MRC National Institute for Medical Research, UK.
  • We love our O2k. We have performed measurements in more than 300 muscle biopsies to date, and Paul Coen and I just got a paper accepted in Journal of Gerontology highlighting the measurements related to function in the elderly. - Bret Goodpaster, MiPNet Lab: US PA Pittsburgh Goodpaster BH.
  • We are so happy with the performance of our two Oroboros machines that we are considering buying a couple more. We tested permeabilized muscle fibers and liver samples, always obtaining an excellent performance. The resolution of the machines goes largely beyond my most optimistic expectations and, so far, I am very positively impressed with their accuracy and experimental possibilities! - Carles Canto, MiPNet Lab: CH Lausanne Canto C.
  • We have been using the Oxygraph-2k non stop since you left Sydney in April. It has been working without issue since then. Today for the first time the chamber A is not stable. I assume that the first thing to do is to polish the electrode.

- Collinson E, Sydney, AU.

  • Thank you for creating such a wonderful apparatus, it made my work so much easier and much more fun. Thanks also for a great course in Schroecken in the spring of 2009, unforgettable! - Persson MF, MipNet Lab: SE Uppsala Liss P.
  • I had not expected that you would send the replacement stoppers before we had sent the others back. This goes above and beyond excellent customer service, you are fantastic! - Christopher Hedges, MiPNet Lab: AU Melbourne Stepto N Victoria University, Australia - Nigel Stepto's MipNet Lab]].


Previous O2k-Feedback

>> Highlights

O2k-Feedback 2011

  • With my very best wishes of a productive 2012 and keep moving on the Oroboros to new horizons, bringing science, personal and life quality as driving forces to a better world. - Marcus Fernandes de Oliveira, Rio de Janeiro, BR (2011-12-29).
  • It is great that you develop the Oxygraph-2k in so wonderful directions.. excellent :))). - Magda Labieniec-Watala, Lodz, PL (2011-11-25).
  • I also just want to mention how happy we are with the O2k respirometer we have and want to congratulate you on the success of your O2k systems (not surprisingly, of couse), hence the already booked December workshop. - Catherine Le, Fort Collins, US (2011-11-11).
  • The first Oroboros in our lab gave good results. We have plans to purchase a second Oroboros. - Daphna D.J. Habets Maastricht, NL (2011-11-04)
  • Actually, we are very satisfied with the results that we are producing with the Oxygraph-2k. - Silvia Cadena, Curitiba, Paraná, Brasil (2011-10-11)
  • We are really satisfied with the O2k and we hope to measure mitochondrial membrane potential ASAP. - Siamak Djafarzadeh, Bern, CH (2011-09-16)
  • Thank you for developing the Oxygraph, it has proved to be an excellent tool and we look forward to upgrading in the near future. - Campbell W. Gourlay, Kent, UK (2011-08-18)
  • Our two Oroboros machines function perfectly, and I have put in a grant for a third machine. - Carsten Lundby, Zurich, CH (2011-08-03)


O2k-Feedback 2010

  • Our O2k runs without problems. - Wolfgang Burgstaller, Innsbruck (2010-11-04)
  • I was participating last summer in WorldPharma 2010 congress in Copenhagen and heard a fantastic presentation given by Professor Sir Salvador Moncada about mitochondrial function measured by OROBOROS Oxygraph. - Eero Mervaala, Helsinki (2010-10-19)
  • Our project is now accepted by the French polar institute (IPEV) and we plan to install a respirometry system in Antarctica! I already worked on the Oroboros in my previous laboratory in Canada and, to my opinion, it’s the best and more accurate system for our project. - Caroline Romestaing, Villeurbanne (2010-09-15)
  • Recently Doug Crawford here in Miami let me use his O2k and it was great. - Carlos Moraes, Miami [email protected] (2010-05-05)
  • The two recently acquired Oxygraphs are now installed and running smoothly. I am very pleased with the new version of Datlab, even though I have not yet had occasion to try out all of the new options added to it. - Ib Therkelsen, Copenhagen, Denmark (2010-03-17) Comment: the Quistorff lab has now a total of five O2k instruments.
  • We´re very happy with our three O2k's here at Karolinska Institute. - Filip Larsen, Stockholm, Sweden (2010-03-05)
  • Happy new year to you and the fantastic Oroboros team! The Oxygraph-2k is working like a dream. - Vinh Thanh Phung, Ås, Norway (2010-01-07)


O2k-Feedback 2009

  • I already start to use this wonderfull instrument. It works really well now! - Arnaud Mourier, Max Planck Institute for Biology of Ageing, Zentrum für Molekulare Medizin, Univ. Köln, Germany (2009-11-16)
  • I came across your website and found excellent information for instrumentation, protocols and solutions. - Steven Dworetzky, Knopp Neurosciences, USA (2009-10-01)
  • .. we are very impressed with the instrument .. - Marni J. Falk, The Children's Hospital of Philadelphia, Philadelphia, USA (2009-08-11)
  • ... For an independent perspective on the OROBOROS Oxygraph-2k, we think they are fantastic, fullstop. We have had no real issues in almost four years. I started with the general system which requires water baths etc and really it was baptism of fire, and when we got the Oroboros-O2k it was a different world. Our only issue now is we need more of them now. Perhaps the best part of our dealings with OROBOROS is Erich's groups outstanding efforts to provide detailed methods and protocols for both the machines and experiments. ... - Anthony J.R. Hickey, University of Auckland, New Zealand (2009-08-07)
  • ... regarding the Seahorse ... they were adament that their instrument was useful for quantitative measurements, even though the vast majority of the data was expressed as fold-change, or % control. In the end, they said that their system was more complimentary to existing oxygraphs, and even suggested that the Seahorse be used for optimization experiments to determine the best conditions for O2k experiments. - Daniel A. Kane, East Carolina University, Greenville, NC, USA (2009-07-30)
  • First of all I would like to write that O2K works very well in our lab as well. - Laszlo Tretter, M.D., PhD. Assoc. Prof., Semmelweis University, Dept. Medical Biochemistry, Budapest, Hungary (2009-07-29)
  • First of all I´d like to say that I´m very happy with the O2k system. I have used it quite a lot lately, mainly doing phosphorylation control titrations with intact podocytes and I have not have any problems, except for a few small ones relating to software. - Ilkka Miinalainen, Ph.D., NICB, Centre for Bioanalytical Sciences, Dublin City University, Ireland (2009-04-27)
  • We continue to "spread the word" about the OROBOROS with our colleagues at meetings and conferences. As with all quality products, I'm not surprised to hear that demand is steady, even in the face of economic hardships. Yours has the added benefit of sophisticated science and precision manufacturing, which exponentially increases its value. Darrell Neufer and I have a big paper being published in JCI on this upcoming Monday (on-line first). There is OROBOROS data all over it. Many more papers are currently being written, as we've been using our O2k's at maximal capacity for the past two years! Would it be possible for you to send a small stack of your "Blue Book of Mitochondria" with the new O2k? It's really an invaluable resource for students and new people that join the lab. - Ethan J. Anderson, Ph.D., Research Associate, Departments of Cardiovascular Sciences/EXSS, Brody School of Medicine, East Carolina University, USA (2009-01-30)
  • Since the two OROBOROS installed here are fully operational (in fact it has been a great success!), the demand for their use has increased susbtantially. Therefore, we are intending to purchase another one. - Marcus F Oliveira, PhD, Instituto de Bioquímica Médica (IBqM), Universidade Federal do Rio de Janeiro, Brasil (2009-01-28)


O2k-Feedback 2008

  • I thought you might be interested to know more about my research and my success stories using your OROBOROS. I investigated mitochondrial capacity in cardiac muscle fibers of a common model of dietary-induced insulin resistance mice and successfully presented parts of my results at the 2008 Annual Obesity Meeting, Phoenix, Arizona. I received so many positive feedbacks on my study. I am planning on developing my area of research from animals to humans, if it would be possible. I am very proud of what I have achieved during the last year. Starting with basic knowledge about mitochondria and OROBOROS, I have been able to find my way through the mitochondria respiratory world. This achievement wouldn’t be possible without your help and support. - Zahra Ezzat Zadeh, University of Calgary, Canada (2008-12-23)
  • The assemblage and tests of O2K have been finished and some trials have been conducted for TCM research. - Canghai Li, China Academy of Chinese Medical Sciences, Beijing, P.R.China (2008-10-23)
  • The second O2K works perfectly. It will really help us to develop more quickly applied respirometry on muscle cell culture and biopsies. - Dominique Votion, Faculty of Veterinary Medicine, University of Liège, Berglium
  • I have several papers in the pipeline that utilize respiration as our primary variable. The Oxygraph-2k was one of the best investments I ever made. - James Bennett, Department of Neurology, University of Virginia, Charlottesville, USA (2008-09-03)
  • The results with the lymphocytes cells are excellent with MiR05 and really better than with the other medium (more stability and higher values). - Virginie Rhein, Neurobiology Lab for Brain Aging and Mental Health, Associated Research Group Dept. Biomedicine Univ. Basel, Psychiatric University Clinics Basel, CH (2008-08-29)
  • I have been studying respiration in cells and in animals, and plan to include mitochondria. Your website will be a valuable resource. My students will find it interesting. - Dennis Searcy, Professor of Biology, University of Massachusetts, USA.
  • SUPERSATISFIED! - Eskil Elmer, Laboratory for Experimental Brain Research, Wallenberg Neuroscience Center, Lund, Sweden (2008-06-25)
  • Our OROBOROS Oxygraph is in almost daily use and we are very pleased with the data it produces. - Paula M. Keeney, Department of Neurology, University of Virginia, Charlottesville, USA.
  • Our new O2k started up perfectly. The new software is fantastic too. - Anthony Hickey, School of Biological Sciences, University of Auckland, New Zealand.
  • I would like to congratulate the Oroboros team for the software that really works beautifully. People who did it are really clever (know how someone using an oxygraph works). - Frédérick Bouillaud, Faculté de Médecine Necker-Enfants Malades, Paris, France.
  • People are getting really happy with the Oroboros ... the instrument is working remarkably well so far. - Rafael Radi, Universidad de La Republica, Montevideo, Uruguay.
  • .. we tested a few techniques using the O2K. I really enjoyed working with the instrument and so far I am very happy with it. - Charles-A. Darveau, Dept. of Biology, University of Ottawa, Canada.


O2k-Feedback 2007

  • .. feedback about the O2K. I am very impressed. The last 3 years I've done many expts. on mito respiration in permeabilized fibers using a Hansatech electrode, which worked fine and was suitable for our needs at the time. But I equate going from that system to the O2K to starting with a Honda Accord and going to an Infiniti or a Mercedes C-class. Once you've had a taste of high-performance, there's no going back. :) - Ethan J. Anderson, East Carolina University, NC, USA.
  • We are loving work with the OROBOROS-O2k, despite we were not using all possibilities yet. But, step by step... - Antonio Galina, Inst. Med. Biochem., Federal Univ. Rio de Janeiro, Brazil.
  • Our Oxygraph-2k is in daily use and is a truly wonderful instrument. I may be forced to buy a second one. - I am very interested in the TPP+ electrode development. Please keep us in mind as a potential "beta" testing site. - James P. Bennett, Center for the Study of Neurodegenerative Diseases, Charlottesville, VA, USA.
  • I have been working with CS using your protocol (Laboratory Protocol Citrate Synthase), which I found to be extremely helpful and very well-written. Thank you, very good job! - Nikolai Chepelev, M.Sc. Biology Student, Carleton University, Ottawa, Canada.
  • Your web page provides the clearest, most concise explanations I have ever read on why mitochondrial substrates are used as they are (glutamate, malate, etc...). - Florian L. Muller, Ph.D., Dept. of Cell and Structural Biology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
  • We had no problems with installation of the Oxygraph-2k. - Olivier Douay, Univ. Hospital Angers, France - (2007-03-27).
  • The O2k arrived Friday last week in excellent condition, IT IS A BEAUTY! We have installed the software and set up the electrodes and everything seems to work nicely. - Eskil Elmér Lund University, Sweden (2007-01-15).


O2k-Feedback 2006

  • See also O2k-course IOC32-Feedback.
  • We are working hard with the excellent Oxygraph-2k. .. I am spreading the good news about your perfect oxygraph. I've made a collegue of mine enthusiastic, ... - Willem Sluiter - [email protected] (2006-10-18).
  • Concerning our three Oxygraph-2k, they are all running smoothly like Swiss clocks (Austrian clocks, if you like...). My college, Morten, who also joined the IOC32 in April 2006, has been using the instruments almost every day through the entire summer for some permeabilised muscle experiments. He reports that all three have been pleasantly stable and well-behaven, and that electrode-membrane replacements have been rare. Only weak spot seems to be the cable connection in the bottom of the electrode holder. The cable does not like to be touched, bent, or moved about during data acquisition. Some stabilisation of the cable entry into the electrode holder might possibly be a point for improvement in later generation design. Last week of August, we took the three instruments to the nearby veterinarian school in order to perform measurements on 40 biopsies from sheep. Those were busy days for all three persons involved, but also quite satisfactory, as there was not a single mishap with either samples or machines. As to the Savitzky-Golay filter [Oxygen Flux and Time Resolution] and two second sampling intervals, we have found that for all experiments performed in our lab up until now, we never needed to do it any differently. We have not yet had the need for rapid transition measurements, but we have tried, once, to run the data acquisition at full pelt, just for the fun of it. - Ib Therkelsen, University of Copenhagen, Denmark (2006-10-17).
  • ... Now my Ok2 is operating soooooooooo well, and the problem of analysis of the mito data has been solved successfully as you said me [Oxygen Flux and Time Resolution]. I reduced the mitochondrial concentration (from 0.5 to 0.3 mg protein/ml ) and I´ve reduced the data recording interval to the minimum of 0.2 s too. After ADP additon the slope increases sharply and returns to state 4 with similar values to State 2 ones, resulting in a higher RCR. - Ana López Ramírez - [email protected] (2006-10-17).
  • Yes the machine is working well. Very well actually. Took a bit of trial and error with the electrolyte, as our water here is full of metal ions. Distilled water did the job. - Anthony J.R. Hickey, University of Auckland, New Zealand (2006-07-13).
  • We are delighted with our O2k! It is obviously a very sensitive piece of equipment and the DatLab software makes using it very easy. The respiratory state of our cell lines will be important information for our group. I must be honest though, with no background in respirometry or with the O2k, I would have been lost without all the very helpful instruction I received in Shrocken. Thank you for making our use of this fine machine possible! - Paula Keeney, Center for the Study of Neurodegenerative Diseases, Charlottesville, USA (2006-06-02).
  • Here we are! We received the Oxygraph-2k on wednesday, we tested it for two days and it workes very well! Great! - Mamen Carmona, Diabetes Laboratory, Barcelona, Spain (2006-02-24).
  • The following review of one of your products has been left by a SelectScience.net member (2006-02-09):
Product: Oxygraph-2k
Ease of use: 5 (top rating)
After sales: 5 (top rating)
Value for money: 5 (top rating)
Overall: 5.0 (top rating)
Comments: Easy to use, with a user-friendly interface. Online results are great!
Name: Rasmus Rabol - [email protected]
Company: University og Copenhagen
Field: Med Physiology


O2k-Feedback 2005

  • On respirometry with permeabilized muscle fibres: .. thanks for your help and lifesaving website and info. - Anthony J.R. Hickey, University of Auckland, New Zealand (2005-12-08).
  • Getting OROBOROS was a natural choice. - Hong Kyu Lee, Seoul National University Hospital, South Korea (2005-11-25).
  • We have an Oroboros-2k which is a perfect machine. On the web-site you will find all material relevant to this machine, including mito isolation protocols and an estimate of the amount of tissue needed. The Oroboros simultaneously runs respirometry in 2 two ml chambers. The large volume is advantageous as this implies your mitos can respire for quite a while without running out of oxygen. As the sensors are fairly sensitive and stable you do not really need much tissue. ... you may consider to run the assays not on isolated mitos but on permeabilized cardiomyocytes (we run analyses on permeabilized muscle biopsies ..). The advantage is that this may need less tissue; .. the machine can do wonderful things. If you decide to buy one I can certainly advise you to run the course Erich offers as it is by far the best way to get some feeling of what can be achieved with the machine. - Matthijs Hesselink, Maastricht University, The Netherlands (2005-11-22).
  • The perspectives of working with the OROBOROS Oxygraph-2k on human tissue are enormous. - Flemming Dela, Department of Medical Physiology, The PANUM Institute, Copenhagen, Denmark (2005-11-21).
  • It is a pleasure to give you the permission to list our lab as an OROBOROS 'Mitochondrial Physiology Network Laboratory'. I do think this kind of network will be very useful for us. - Louis Lefaucheur, Agrocampus Rennes Systèmes d'Elevage et Nutrition Animale et Humaine, Saint-Gilles, France (2005-10-27)
  • Polarography is a technique that we have been using routinely in the lab for mitochondrial research or the diagnosis of mitochondrial diseases, and so far the Oroboros Oxygraph is the best in terms of sensitivity and reproducibility. Respiratory studies with cells give good results with about 5 10E5 cells/ml , and depending on the cell type you can go down to 1E5. We used primary myoblasts, keratinocytes, lymphocytes and a variety or cancer cells, permeabilized or not. You can also utilize permeabilized muscle fibers of about 3 mg each. The good point is that you can measure the oxygen consumption of the electrode (noise) and the oxygen diffusion inside the machine in a pre-experiment, that you remove from your respiration data. For kinetic studies you can also take into account the time constant response of the electrode (that increases with time) that can also be measured. What must be pointed out, is that this Oxygraph is the only one that allows to work at very low (physiological and even hypoxic) oxygen concentrations, what cannot be performed with classic systems that are not oxygen-proof enough. Also, the software is powerful and the new version more user-friendly as older versions. Recently, this company developed a new stopper that allows to fit in electrodes or optic fibers that allow to measure bioenergetic or cellular parameters concomitantly to oxygen consumption rate. - Rodrigue Rossignol (MIG list circular 2005-04-28).
  • I have been working with the Oroboros Oxygraph-2k since two years now, and concerning the sensitivity I can say, that I obtained reliable results even with cell numbers of about 0.1 Mill cells/ml, which would correspond to a cellular protein concentration of about 0.3 mg/ml. - Evi Hütter (MIG list circular 2005-04-28).
  • I have been using the Oxygraph-2k for two years and due to its sensitivity I can use it to measure the respiration of intact fibroblasts from one 75 sq.cm culture flask - that could be like 0.2 mg/ml of protein and I guess it might be possible to go even lower with the protein concentration. With isolated mitochondria, measurements at 0.025 mg/ml are feasible. I must say I am very satisfied with the instrument, but frankly I have never used any other oxygen electrode so my opinion is not unbiased. - Petr Pecina (MIG list circular 2005-04-27).


O2k-Feedback 2004

  • Your new machine is amazingly confortable to work with. Time constants are really good and the new software used to drive the machine is more convenient than the previous DatLab 2 acquisition program ! We have very nice results on skinned fibers from pig or rat heart. Signals and biological samples are really stable over a large time scale, which is needed to perform accurate ADP titrations. We would like to buy another OROBOROS Oxygraph 2k. This would allow two persons of the lab to perform large scale and simultaneous studies on isolated mitochondria, myocardic cells and skinned fibers. - Bertrand Beauvoit (2004-07-07).
  • This is to express our sincere gratitude for the course last weekend. We found the course highly instructive and fruitful and have enjoyed the relaxed atmosphere very much. - Matthijs Hesselink (2003-12-16).
  • The OROBOROS precision for O2 concentration measurements is 0.036 microM against 0.500 to 1.000 microM for other commercial instruments. - Grégori G, Denis M, Lefèvre D, Beker B (2002).


O2k-Feedback 2003

  • Hello Erich: The O2k-Workshop was again exquisite and informative, and your knowledge begs those who yearn to learn. Interesting how many ideas within the talent of those gathered, young and senior, create an atmosphere for discovery. Also, thank you for all your altruistic help with my projects. - Robert Boushel, Montreal, Canada/Copenhagen, Denmark (2003-12-17).
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