Noise: Difference between revisions
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|description=In [[fluorometry]] and [[spectrophotometry]], '''noise''' can be attributed to the statistical nature of the photon emission from a [[light source]] and the inherent noise in the instrument’s electronics. The former causes problems in measurements involving samples of analytes with a low [[extinction coefficient]] and present only in low concentrations. The latter becomes problematic with high [[absorbance]] samples where the light intensity emerging from the sample is very small. | |description=In [[fluorometry]] and [[spectrophotometry]], '''noise''' can be attributed to the statistical nature of the photon emission from a [[light source]] and the inherent noise in the instrument’s electronics. The former causes problems in measurements involving samples of analytes with a low [[extinction coefficient]] and present only in low concentrations. The latter becomes problematic with high [[absorbance]] samples where the light intensity emerging from the sample is very small. | ||
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{{MitoPedia methods}} | {{MitoPedia methods | ||
|mitopedia method=Fluorometry, Spectrophotometry | |||
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{{MitoPedia topics}} | {{MitoPedia topics}} | ||
Revision as of 17:36, 25 November 2011
- high-resolution terminology - matching measurements at high-resolution
Noise
Description
In fluorometry and spectrophotometry, noise can be attributed to the statistical nature of the photon emission from a light source and the inherent noise in the instrument’s electronics. The former causes problems in measurements involving samples of analytes with a low extinction coefficient and present only in low concentrations. The latter becomes problematic with high absorbance samples where the light intensity emerging from the sample is very small.
Abbreviation: n.a.
MitoPedia methods:
Fluorometry,
Spectrophotometry
