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Difference between revisions of "NextGen-O2k Scientific developments"

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== Progress and next steps ==
== Progress and next steps ==
:::: '''2020-10-08:''' Laurent Beck started with measurements on ''Chlamydomonas reinhardtii'' using the PB- and Q-Modules , supervised by [[Huete-Ortega Maria|PI Maria Huete-Ortega]] and Thomas Roach (University of Innsbruck, Institute of Botany)
:::: '''2021-02-18:''' MitoFit preprint publication: [[Komlodi 2021 MitoFit Q|Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]]
:::: '''2021-01-29:''' Deliverable 2.1 submitted.
:::: '''2021-01-29:''' MitoFit preprint publication: [[Cardoso 2021 MitoFit MgG|Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration]]
:::: '''2020-10-08:''' Laurent Beck started with measurements on ''Chlamydomonas reinhardtii'' using the PB- and Q-Modules , supervised by [[Huete-Ortega Maria|PI Maria Huete-Ortega]] and Thomas Roach (University of Innsbruck, Institute of Botany)
:::: '''2020-07-23:''' First isolation of mitochondria in microalgae trials
:::: '''2020-07-23:''' First isolation of mitochondria in microalgae trials
:::: '''2020-07-01:''' Start of the development of protocols for permeabilizing microalgae cells
:::: '''2020-07-01:''' Start of the development of protocols for permeabilizing microalgae cells
:::: '''2020-04-24:''' First tests with pH electrodes
:::: '''2020-04-24:''' First tests with pH electrodes
:::: '''2020-03-11:''' First tests with [[Calcium Green]]
:::: '''2020-03-11:''' First tests with [[Calcium Green]]
:::: '''2020-02-20:''' Started development of protocols (and DL-Protocols) for the PB-Module, including new [[SUIT]] protocols for photosynthesis measurements.
:::: '''2020-02-20:''' Started development of protocols (and DL-Protocols) for the PB-Module, including new [[SUIT]] protocols for photosynthesis measurements.
:::: '''2019-12-31:''' Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
:::: '''2019-12-31:''' Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
:::: '''2019-12-06:''' First version of the Cyclic Voltammetry Manual released: [[MiPNet24.16 DatLab8.0: CV-Module |CV-Module]]
:::: '''2019-12-06:''' First version of the Cyclic Voltammetry Manual released: [[MiPNet24.16 DatLab8.0: CV-Module |CV-Module]]
:::: '''2019-12-02:''' Optimization of O2k-Procedures for isolated mitochondria preparations
:::: '''2019-12-02:''' Optimization of O2k-Procedures for isolated mitochondria preparations
:::: '''2019-11-22:''' First version of NextGen-O2k Manual/SOP finalized: [[MiPNet24.12 NextGen-O2k: Q-Module |NextGen-O2k: Q-Module]]
:::: '''2019-11-22:''' First version of NextGen-O2k Manual/SOP finalized: [[MiPNet24.12 NextGen-O2k: Q-Module |NextGen-O2k: Q-Module]]
:::: '''2019-11-21:''' Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
:::: '''2019-11-21:''' Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
:::: '''2019-11-18:''' Application of Q-redox state measurements in scientific projects
:::: '''2019-11-18:''' Application of Q-redox state measurements in scientific projects
:::: '''2019-10-22:''' 66 [[SUIT]] protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see [[MitoPedia:_SUIT]]
:::: '''2019-10-22:''' 66 [[SUIT]] protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see [[MitoPedia:_SUIT]]
:::: '''2019-10-21:''' Four new videos for DatLab 7.4 software released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#DatLab_7.4 O2k-Videosupport DatLab 7.4]
:::: '''2019-10-21:''' Four new videos for DatLab 7.4 software released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#DatLab_7.4 O2k-Videosupport DatLab 7.4]
:::: '''2019-09-24:''' Two new videos for mitochondrial preparations released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#O2k-Mitochondrial_preparations O2k-Videosupport Mitochondrial preparations]
:::: '''2019-09-24:''' Two new videos for mitochondrial preparations released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#O2k-Mitochondrial_preparations O2k-Videosupport Mitochondrial preparations]
:::: '''2019-09-23:''' First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1<sup>st </sup>Q-Module workshop at the [[MiPNet24.02 IOC141 Schroecken AT |Oroboros O2k-Workshop IOC141]]
:::: '''2019-09-23:''' First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1<sup>st </sup>Q-Module workshop at the [[MiPNet24.02 IOC141 Schroecken AT |Oroboros O2k-Workshop IOC141]]
:::: '''2019-09-20:''' First six videos for the Q-Module released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport Q-Module]
:::: '''2019-09-20:''' First six videos for the Q-Module released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport Q-Module]
:::: '''2019-09-06:''' Development of two harmonized [[SUIT]] protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
:::: '''2019-09-06:''' Development of two harmonized [[SUIT]] protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
:::: '''2019-07-17:''' First tests of the new [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| O2 analysis template DL7.4]]
:::: '''2019-07-17:''' First tests of the new [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| O2 analysis template DL7.4]]
:::: '''2019-07:''' Started development of [[SUIT]] protocols for isolated mitochondria and permeabilzied cells
:::: '''2019-07:''' Started development of [[SUIT]] protocols for isolated mitochondria and permeabilzied cells
:::: '''2019-06-18:''' Testing of the [[SUITbrowser]] by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), [[MiPNet24.01 IOC139 Schroecken AT| IOC139]]
:::: '''2019-06-18:''' Testing of the [[SUITbrowser]] by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), [[MiPNet24.01 IOC139 Schroecken AT| IOC139]]
:::: '''2019-06:''' Integration of cyclic voltammetry as a quality control step into the Q-Module
:::: '''2019-06:''' Integration of cyclic voltammetry as a quality control step into the Q-Module

Revision as of 10:28, 22 February 2021

Template:MitoFit highlights page name

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP2: Scientific developments



Abstract

The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
  • Determination of optimal Q-sensor measurement conditions
  • Determination of optimal measurement conditions for the PB-Module
  • Development of protocols for scientific, pre-clinical and algal biotechnology applications
  • Data handling and quality control

Achievements

Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.

Progress and next steps

2021-02-18: MitoFit preprint publication: Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria
2021-01-29: Deliverable 2.1 submitted.
2021-01-29: MitoFit preprint publication: Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration
2020-10-08: Laurent Beck started with measurements on Chlamydomonas reinhardtii using the PB- and Q-Modules , supervised by PI Maria Huete-Ortega and Thomas Roach (University of Innsbruck, Institute of Botany)
2020-07-23: First isolation of mitochondria in microalgae trials
2020-07-01: Start of the development of protocols for permeabilizing microalgae cells
2020-04-24: First tests with pH electrodes
2020-03-11: First tests with Calcium Green
2020-02-20: Started development of protocols (and DL-Protocols) for the PB-Module, including new SUIT protocols for photosynthesis measurements.
2019-12-31: Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
2019-12-06: First version of the Cyclic Voltammetry Manual released: CV-Module
2019-12-02: Optimization of O2k-Procedures for isolated mitochondria preparations
2019-11-22: First version of NextGen-O2k Manual/SOP finalized: NextGen-O2k: Q-Module
2019-11-21: Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
2019-11-18: Application of Q-redox state measurements in scientific projects
2019-10-22: 66 SUIT protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see MitoPedia:_SUIT
2019-10-21: Four new videos for DatLab 7.4 software released - see: O2k-Videosupport DatLab 7.4
2019-09-24: Two new videos for mitochondrial preparations released - see: O2k-Videosupport Mitochondrial preparations
2019-09-23: First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1st Q-Module workshop at the Oroboros O2k-Workshop IOC141
2019-09-20: First six videos for the Q-Module released - see: O2k-Videosupport Q-Module
2019-09-06: Development of two harmonized SUIT protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
2019-07-17: First tests of the new O2 analysis template DL7.4
2019-07: Started development of SUIT protocols for isolated mitochondria and permeabilzied cells
2019-06-18: Testing of the SUITbrowser by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139
2019-06: Integration of cyclic voltammetry as a quality control step into the Q-Module
2019-06: Testing of the SUITbrowser

Links and references