- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-substrates of S-respiration. In typical SUIT protocols with type N and S substrates, flux under NS-pathway control, NS, is inhibited by Rotenone to measure flux under S-pathway control, SRot or S. Then the NS-S control factor is
jNS-S = (NS-S)/NS
The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux under S-pathway control in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jNS-S
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
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