- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-substrates of S-respiration. In typical SUIT protocols with type N and S substrates, flux in the NS-substrate state, NS, is inhibited by Rotenone to measure flux in the S-substrate state, S. Then the NS-S control factor is
jNS-S = (NS-S)/NS
The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux in the S-substrate state in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-substrate background state does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jNS-S
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Compare
