NS-S pathway control efficiency: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''j''<sub>NS-S</sub> | |abbr=''j''<sub>NS-S</sub> | ||
|description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-substrates of S-respiration. In typical [[SUIT protocol]]s with [[ETS substrate types |type N and S substrates]], flux | |description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-substrates of S-respiration. In typical [[SUIT protocol]]s with [[ETS substrate types |type N and S substrates]], flux under [[NS-pathway control]], NS, is inhibited by [[Rotenone]] to measure flux under [[S-pathway control]], SRot or S. Then the NS-S control factor is | ||
''j''<sub>NS-S</sub> = (NS-S)/NS | ''j''<sub>NS-S</sub> = (NS-S)/NS | ||
The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux | The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux under S-pathway control in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | ||
|info=[[Flux control factor]] | |info=[[Flux control factor]] | ||
}} | }} | ||
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|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
== Compare == | == Compare == | ||
::::* [[CII control ratio]] | ::::* [[CII control ratio]] |
Revision as of 15:59, 26 August 2016
- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-substrates of S-respiration. In typical SUIT protocols with type N and S substrates, flux under NS-pathway control, NS, is inhibited by Rotenone to measure flux under S-pathway control, SRot or S. Then the NS-S control factor is
jNS-S = (NS-S)/NS
The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux under S-pathway control in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jNS-S
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Compare