Difference between revisions of "NS-S pathway control efficiency"

Revision as of 15:59, 26 August 2016

high-resolution terminology - matching measurements at high-resolution

NS-S pathway control efficiency

Description

The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-substrates of S-respiration. In typical SUIT protocols with type N and S substrates, flux under NS-pathway control, NS, is inhibited by Rotenone to measure flux under S-pathway control, SRot or S. Then the NS-S control factor is

j_NS-S = (NS-S)/NS

The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux under S-pathway control in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).

Abbreviation: j_NS-S

Reference: Flux control factor

MitoPedia concepts: Respiratory control ratio

MitoPedia methods: Respirometry

Compare

CII control ratio

List of publications: CI&II and CII

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=''j''<sub>NS-S</sub>
-|description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-substrates of S-respiration. In typical [[SUIT protocol]]s with [[ETS substrate types |type N and S substrates]], flux in the [[NS-substrate state]], NS, is inhibited by [[Rotenone]] to measure flux in the [[S-substrate state]], S. Then the NS-S control factor is
+|description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-substrates of S-respiration. In typical [[SUIT protocol]]s with [[ETS substrate types |type N and S substrates]], flux under [[NS-pathway control]], NS, is inhibited by [[Rotenone]] to measure flux under [[S-pathway control]], SRot or S. Then the NS-S control factor is
   ''j''<sub>NS-S</sub> = (NS-S)/NS
-The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux in the S-substrate state in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-substrate background state does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII).
+The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux under S-pathway control in the presence of a type N substrate combination. Experimentally rotenone (Rot) is added to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII).
 |info=[[Flux control factor]]
 }}
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 |mitopedia method=Respirometry
 }}
-{{MitoPedia O2k and high-resolution respirometry}}
-{{MitoPedia topics}}
 == Compare ==
 ::::* [[CII control ratio]]