Difference between revisions of "NS-S pathway control efficiency"
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|description=The '''CI<small>&</small>II-CII [[substrate control factor]]''' expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from [[CII-linked respiration]] ([[succinate]]) in the presence of a CI-linked substrate combination. Experimentally rotenone (Rot) is added to [[CI&II-linked respiration]]. The reversed protocol, adding CI-linked substrates to a CII-linked background state does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | |description=The '''CI<small>&</small>II-CII [[substrate control factor]]''' expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from [[CII-linked respiration]] ([[succinate]]) in the presence of a CI-linked substrate combination. Experimentally rotenone (Rot) is added to [[CI&II-linked respiration]]. The reversed protocol, adding CI-linked substrates to a CII-linked background state does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | ||
|info=[[Flux control factor]] | |info=[[Flux control factor]] | ||
}} | |||
{{MitoPedia concepts | |||
|mitopedia concept=Respiratory control ratio | |||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia O2k and high-resolution respirometry}} | ||
{{MitoPedia topics}} | |||
}} | |||
== Compare == | == Compare == | ||
* [[CII control ratio]] | * [[CII control ratio]] | ||
Revision as of 15:06, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The CI&II-CII substrate control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from CII-linked respiration (succinate) in the presence of a CI-linked substrate combination. Experimentally rotenone (Rot) is added to CI&II-linked respiration. The reversed protocol, adding CI-linked substrates to a CII-linked background state does not provide a valid estimation of CII-linked respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jCI&II-CII
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Compare
