- high-resolution terminology - matching measurements at high-resolution
NADH electron transfer-pathway state
Description
NADH-linked substrates (CI-linked) are type N substrates of ETS-level 4, feeding electrons into the N-junction catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrates individually or in combination: pyruvate, glutamate, malate, oxoglutarate, citrate, 2-oxoglutarate, hydroxybutyrate. These type N substrates are (indirectly) linked to Complex I by the corresponding dehydrogenase-catalyzed reactions reducing NAD+ to NADH+H+. In mt-preparations, succinate dehydrogenase (SDH; CII) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the pesence of type N substrates can be further suppressed by the CII-inhibitor malonic acid).
Abbreviation: N
Reference: N-junction
MitoPedia concepts:
Respiratory state,
SUIT state
Contributed by Gnaiger E 2016-03-20; edited 2016-08-26.
