NADH electron transfer-pathway state

high-resolution terminology - matching measurements at high-resolution

NADH electron transfer-pathway state

Description

NADH-linked substrates (CI-linked) are type N substrates of ETS-level 4, feeding electrons into the N-junction catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrates individually or in combination: pyruvate, glutamate, malate, oxoglutarate, citrate, hydroxybutyrate. These type N substrates are (indirectly) linked to Complex I by the corresponding dehydrogenase-catalyzed reactions reducing NAD⁺ to NADH+H⁺. In mt-preparations, succinate dehydrogenase (SDH; CII) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the pesence of type N substrates can be further suppressed by the CII-inhibitor malonic acid).

Abbreviation: N

Reference: N-junction

MitoPedia concepts: Respiratory state 

MitoPedia O2k and high-resolution respirometry: "SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT state"SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.