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| {{MitoPedia
| | #REDIRECT [[Nicotinamide adenine dinucleotide]] |
| |abbr=NADH
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| |description='''Nicotinadeninedinucleotide (NADH)''' is not permeable through the inner mt-membrane. The NADH pool integrates the activity of the [[TCA cycle]] and various matrix dehydrogenases upstream of [[CI]], and thus forms a junction or funnel of electron transfer to CI. Cytosolic NADH is effectively made available for mitochondrial respiration through the [[malate-aspartate shuttle]] or [[Glycerophosphate_dehydrogenase_complex|glycerophosphate dehydrogenase]].
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| |type=Substrate ETS
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| {{MitoPedia methods|type=Substrate ETS
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| {{MitoPedia topics
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| |mitopedia topic=Substrate and metabolite
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| |type=Substrate ETS
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| {{Labeling
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| |enzymes=Complex I
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| == Application in [[HRR]] ==
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| '''n: Nicotinadeninedinucleotide, NADH''' (β-Nicotinamide adenine dinucleotide, reduced potassium salt, C<sub>21</sub>H<sub>27</sub>N<sub>7</sub>O<sub>14</sub>P<sub>2</sub>K<sub>2</sub>); Sigma A 4505, 100 mg, store at -20 °C (old Sigma recommendation : 4-8°); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n4505pis.pdf]. The same precautions are recommended by Sigma for the sodium salt.
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