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Difference between revisions of "Mitochondrial preparations"

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{{MitoPedia
{{MitoPedia
|abbr=mt-preparations
|abbr=mtprep
|description='''mt-preparations''' are isolated mitochondria (Imt), submitochondrial particles (Smp), tissue homogenate (Tho), mechanically or chemically permeabilized tissue (permeabilized fibres, Pfi) or permeabilized cells (Pc). In these preparations the cell membranes are either removed (Imt and Smp) or mechanically (Tho) and chemically permeabilized (Pfi).
|description='''Mitochondrial preparations''' (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent mt-structure are maintainedΒ  in incubation media optimized to support mitochondrial physiological performance. According to this definition, submitochondrial particles (smtp) are not a mtprep, since mitochondrial structure is altered although specific mitochondrial functions are preserved.
|type=Respiration
}}
{{MitoPedia methods|type=Respiration
}}
{{MitoPedia topics
|mitopedia topic=Sample preparation
|type=Respiration
}}
}}
Communicated by [[Gnaiger E]] 2011-11-15 (last edit 2022-12-11).
== MitoPedia: mt-preparations ==
== MitoPedia: mt-preparations ==


* [[Isolated mitochondria]], Imt ([[Gnaiger 2009 Int J Biochem Cell Biol]])
::::* [[Isolated mitochondria]], imt
* [[Submitochondrial particles]], Smp
::::* [[Tissue homogenate]], thom
* [[Tissue homogenate]], Thom
::::* [[Permeabilized tissue]], pti
* [[Permeabilized tissue or cells]], Ptic
::::* [[Permeabilized muscle fibers]], pfi
* [[Permeabilized muscle fibres]], Pfi ([[Gnaiger 2009 Int J Biochem Cell Biol]])
::::* [[Permeabilized cells]], pce
* [[Permeabilized cells]], Pc


'''O2k Protocols for mt-preparations:''' [[O2k-mitochondrial preparations]]
::::Β» Compare: [[Submitochondrial particles]], smtp


:::: '''O2k-Protocols for mt-preparations:''' [[O2k-mitochondrial preparations]]


== References ==


{{#ask:[[Additional label::Mitochondrial preparations]]
| mainlabel=Bioblast link
|?Has title=Reference
|?Was published in year=Year
|format=broadtable
|limit=5000
|offset=0
|sort=Has title
|order=ascending
}}


{{Labeling
{{Labeling
|preparations=Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate
|preparations=Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate
}}
{{MitoPedia topics
|mitopedia topic=Sample preparation
}}
}}

Latest revision as of 13:29, 11 December 2022


high-resolution terminology - matching measurements at high-resolution


Mitochondrial preparations

Description

Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent mt-structure are maintained in incubation media optimized to support mitochondrial physiological performance. According to this definition, submitochondrial particles (smtp) are not a mtprep, since mitochondrial structure is altered although specific mitochondrial functions are preserved.

Abbreviation: mtprep

Communicated by Gnaiger E 2011-11-15 (last edit 2022-12-11).

MitoPedia: mt-preparations

Β» Compare: Submitochondrial particles, smtp
O2k-Protocols for mt-preparations: O2k-mitochondrial preparations

References

Bioblast linkReferenceYear
Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v12020


Labels:



Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate 





MitoPedia topics: Sample preparation