Difference between revisions of "MitoSOX"
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::::• Rapidly and selectively '''targeted to the mitochondria''' | ::::• Rapidly and selectively '''targeted to the mitochondria''' | ||
'''MitoSOX<sup>TM</sup>''' has been widely used in life cell imaging but it is not free of problems and should be used cautiously. For example, [[Polster 2014 Methods Enzymol]], highlighted that the use of potentiometric dyes which accumulates into the mitochondria due to its moiety with [[TPP+]], confers a membrane potential sensitivity that creates a series of artifacts and problems not often considered. | '''MitoSOX<sup>TM</sup>''' has been widely used in life cell imaging but it is not free of problems and should be used cautiously. For example, [[Polster 2014 Methods Enzymol]], highlighted that the use of potentiometric dyes which accumulates into the mitochondria due to its moiety with [[TPP<sup>+</sup>]], confers a membrane potential sensitivity that creates a series of artifacts and problems not often considered. | ||
|type=General | |type=General | ||
}} | }} | ||
Revision as of 13:12, 18 September 2018
- high-resolution terminology - matching measurements at high-resolution
MitoSOX
Description
MitoSOXTM is the version of the hydroetidine design to target mitochondria in live cells for the detection of anion superoxide (O2-°). The oxidation of the compund by O2-° is easily detected in the red spectrum. One of the advantages of MitoSOXTM is its selectivity for O2-° but not for other Reactive oxygen species or Reactive nitrogen species.
- • Readily oxidized by superoxide but not by other ROS- or RNS-generating systems
- • Absorption/emission maxima: ~510/580 nm
- • Use for live cell imaging
- • Rapidly and selectively targeted to the mitochondria
MitoSOXTM has been widely used in life cell imaging but it is not free of problems and should be used cautiously. For example, Polster 2014 Methods Enzymol, highlighted that the use of potentiometric dyes which accumulates into the mitochondria due to its moiety with [[TPP+]], confers a membrane potential sensitivity that creates a series of artifacts and problems not often considered.
