From Bioblast
MiPNet21.11 IOC116 Innsbruck AT
 Innsbruck AT, 2016 Nov 21-25. OROBOROS O2k-Workshop on O2k-Fluorometry - advanced, IOC116. |
OROBOROS (2016-11-21) Mitochondr Physiol Network
Abstract:
OROBOROS O2k-Workshop on O2k-Fluorometry - advanced. Innsbruck, Austria; 2016 Nov 21-25.
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
ORO, IOC, 2016, MitoFit, Next
Programme
| Date | Time | Topic |
|---|---|---|
| Mo Nov 21 | Arrival | |
| Tu Nov 22 | 08:30 - 10:30 | Introduction O2k-Fluorometry: mt-membrane potential and H2O2 production. O2k-Fluo demo experiment: H2O2 production in permeabilized HEK cells. |
| 10:30 | Coffe/tea break | |
| 10:45 - 12:30 | Hands-on experiment: Respiration and H2O2 production in permeabilized HEK cells. | |
| 12:30 | Lunch | |
| 14:00 - 16:00 | DatLab analysis and collection of experimental data. | |
| 16:00 | Coffee/tea break | |
| 16:15 - 17:30 | Data discussion, SUIT protocol interpretation, summary. | |
| 18:00 | Dinner | |
| We Nov 23 | 08:30 - 10:30 | O2k-potentiometric measurement of mt-membrane potential with TPP+: demo with mouse brain isolated mitochondria. |
| 10:30 | Coffe/tea break | |
| 10:45 - 12:30 | Data analysis. | |
| 12:30 | Lunch | |
| 14:00 - 16:00 | Hands-on: TPP+ or O2k-Fluorometry: Safranin or TMRM. | |
| 16:00 | Coffee/tea break | |
| 16:15 - 17:30 | Data analysis and discussion. | |
| 18:00 | Dinner | |
| Th Nov 24 | 08:30 - 10:30 | O2k-Fluo measurement of phosphorylation of ADP to ATP using Mg-Green: introduction and demo experiment with isolated mitochondria. |
| 10:30 | Coffe/tea break | |
| 10:45 - 12:30 | Hands-on experiment: O2k-Fluo measurement (Mg-Green) of the ~P/O2 ratio in isolated mitochondria. | |
| 12:30 | Lunch | |
| 14:00 - 16:00 | Data analysis and summary of experimental results. | |
| 16:00 | Coffee/tea break | |
| 16:15 - 17:30 | Visit the MitoFit Research Laboratory and reception at OROBOROS INSTRUMENTS. | |
| 18:00 | Dinner | |
| Fr Nov 25 | Departure |
General information
- This O2k-Workshop for advanced users provides a short introduction to HRR. The O2k-Fluo LED2-Module and O2k-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin, TMRM), ATP production (Mg green), and Ca2+ (Ca green) will be discussed.
- » Information on travel and venue: IOC Innsbruck.
Registration
- Please fill in the registration form and send it to [email protected]
- » Registration pdf
Support
- IOC student scholarships are available for this IOC. More information? See here.
Team and guest tutors
- Gnaiger Erich, CEO, OROBOROS INSTRUMENTS and Medical University of Innsbruck, D. Swarovski Research Laboratory
- Sumbalova Zuzana, Principal Investigator (PI), Mitochondrial Physiology Specialist, OROBOROS INSTRUMENTS
- Chinopoulos Christos, Department of Medical Biochemistry, Semmelweis University, Budapest (HU)
Participants
| Participant | Institution | |
|---|---|---|
| Aparicio Trejo Omar Emiliano | MX Mexico City Pedraza Chaverri J: Department of Biology, University of Mexico, Mexico City (MX) | |
|
Cecatto Cristiane | BR Porto Alegre Souza DOG: Universidade Federal do Rio Grande do SuL, Porto Alegre(BR) |
| Einer Claudia | DE Munich Zischka H: Helmholtz Centre Munich (DE) | |
| Grewal Rekha | DE Giessen Eckert GP: Department of Nutrition in Prevention & Therapy, Justus Liebig University, Giessen (DE) | |
| Goy Christine | DE Duesseldorf Haendeler J: Leibniz Research Institute for Environmental Medicine, Molecular Aging Research, Duesseldorf (DE) | |
|
Casado Pinna M | ES Valencia Casado Pinna M: Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia (ES) |
| Hey-Mogensen Martin | US WA Seattle Hey-Mogensen M: Novo Nordisk Research Center, Seattle (US) | |
|
Krizova Jana | CZ Prague Zeman J: Charles University and General University Hospital in Prague (CZ) |
| Matsumoto Junichi | JP Sapporo Yokota T: Department of Cardiovascular Medicine, Hokkaido University, Sapporo (JP) | |
| Moore Anthony | UK Brighton Moore AL: University of Sussex, Brighton (UK) | |
|
Morato Fornaguera Laia | CH Lausanne Sandi C: EPFL, Lausanne (CH) |
| Nair Syam | SE Gothenburg Hagberg H: Institute of Neuroscience and Physiology, University of Gothenburg (SE) | |
| Nielsen Birgitte | DK Copenhagen Poulsen HE: Rigshospitalet Copenhagen, Lab of clinical Pharamacology (DK) | |
| Jit Poddar | IN Haldia Chakrabarti S: ICARE Institute of Medical Sciences and Research, Haldia (IN) | |
| Schaefer Carmina | DE Giessen Eckert GP: Department of Nutrition in Prevention & Therapy, Justus Liebig University, Giessen (DE) | |
| Spinazzi Marco | BE Leuven Spinazzi M: KU Leuven Center for Human, VIB Center for the Biology of Disease (BE) | |
| Weir Mary | CA Antigonish Kane DA: St. Francis Xavier University, Antigonish (CA) | |
| Wyss Rahel | CH Bern Longnus S: Bern University Hospital, Department of Cardiovascular Surgery (CH) |
O2k perspectives - abstracts
- Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.
- » Abstract guidelines
no abstracts found
Contact
- OROBOROS INSTRUMENTS
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- O2k - tested and trusted
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops are listed as MitoGlobal Events.
