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MiPNet21.11 IOC116 Innsbruck AT

Innsbruck AT, 2016 Nov 21-25. OROBOROS O2k-Workshop on O2k-Fluorometry - advanced, IOC116.

OROBOROS (2016-11-21) Mitochondr Physiol Network

Abstract:

OROBOROS O2k-Workshop on O2k-Fluorometry - advanced. Innsbruck, Austria; 2016 Nov 21-25.

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Programme

Monday Nov 21

Time	Topic
	Arrival
18:00	Dinner
19:30	Get-together and introduction of participants and their research interests - a welcome by OROBOROS INSTRUMENTS.

Tuesday Nov 22

Time	Topic
08:30	Erich Gnaiger Introduction O2k-Fluorometry: mt-membrane potential and H2O2 production.
09:30	Carolina Doerrier, Erich Gnaiger O2k-Fluo demo experiment: H2O2 production in permeabilized HEK cells.
10:30	Coffe/tea break
10:45	Carolina Doerrier Hands-on experiment: Respiration and H2O2 production in permeabilized HEK cells.
12:30	Lunch
14:00	DatLab analysis and collection of experimental data.
15:30	Anthony Moore Investigations into the multi-faceted roles of the alternative oxidases: are they a reflection of the nature of the hydrophobic cavity?
16:00	Coffee/tea break
16:15	OROBOROS-Team and Guest Tutors Data discussion, SUIT protocol interpretation, summary.
18:00	Dinner
19:30	O2k-perpectives presentations

Wednesday Nov 23

Time	Topic
08:30	Zuzana Sumbalova O2k-potentiometric measurement of mt-membrane potential with TPP+: TPP+ calibration and demo with mouse brain isolated mitochondria.
10:30	Coffe/tea break
10:45	Demo with mouse brain isolated mitochondria.
12:30	Lunch
14:00	Hands-on: TPP+ or O2k-Fluorometry: Safranin or TMRM.
16:00	Coffee/tea break
16:15	Zuzana Sumbalova Data analysis and discussion.
18:00	Dinner
19:30	O2k-perpectives presentations

Thursday Nov 24

Time	Topic
08:30	Christos Chinopoulos Determination of ADP-ATP exchange rate in isolated mitochondria.
10:30	Coffe/tea break
10:45	Christos Chinopoulos Demo experiment: O2k-Fluo measurement (Mg-Green) of the ~P/O2 ratio in isolated mitochondria.
12:30	Lunch
14:00	Christos Chinopoulos Data analysis and summary of experimental results.
16:00	Coffee/tea break
16:30	Glühwein at the Christkindl market in the Old Town.
18:00	Dinner
	Departure, Friday Nov 25

Abstracts

	Has title
Komlodi 2016 Abstract IOC116	Komlodi T, Geibl F, Sassani M, Adam-Vizi V, Tretter L (2016) The effect of ΔΨ_mt and ΔpH on mitochondrial reactive oxygen species production - A comparative study using mitochondria isolated from different mammalian tissues. Mitochondr Physiol Network 21.11
Cecatto 2016 Abstract IOC116	Cecatto C, Silva JC, Wajner A, Godoy KS, Ribeiro, RT, Amaral AU, Wajner M (2016) cis-4-Decenoic and decanoic acids impair mitochondrial energy, redox and Ca²⁺ homeostasis and induce mitochondrial permeability transition pore opening in rat brain and liver. Mitochondr Physiol Network 21.11
Poddar 2016 Abstract IOC116	Poddar J, Banerjee A, Mukherjee A, Chakrabarti S (2016) Effects of glucose hypometabolism on mitochondrial functions in rat brain and in SHSY5Y cells in culture. Mitochondr Physiol Network 21.11
Sobotka 2016 Abstract IOC116	Sobotka O, Kucera Otto, Stankova P, Endlicher R, Nozickova K, Banni A, Cervinkova Z (2016) Mitochondrial respiration in fatty liver. Mitochondr Physiol Network 21.11
Nielsen 2016 Abstract IOC116	Nielsen B, Woertwein G, Cejvanovic V, Hansen AR, Weimann A, Bjerring PN, Poulsen HE, Joergensen MB (2016) The function of mitochondria and oxidative stress after antidepressants treatment in rats. Mitochondr Physiol Network 21.11
Krizova 2016 Abstract IOC116	Krizova J, Macakova M, Bohuslavova B, Klima J, Ellederova Z, Zeman J, Motlik J, Hansikova H (2016) Complex I-dependent respiration decline is a marker of Huntington`s disease in spermatozoa of transgenic minipig model. Mitochondr Physiol Network 21.11
Morato 2016 Abstract IOC116	Morato L, Guillot de Suduiraut MI, Grosse J, Zanoletti O, Riccio O, Fournier C, Sandi C (2016) Impaired mitochondrial function mediates early life stress-induced depression. Mitochondr Physiol Network 21.11
Wyss 2016 Abstract IOC116	Wyss RK, Mendez-Carmona N, Nieves Sanz M, Tevaearai Stahel HT, Longnus SL (2016) Tolerance of cardiac mitochondria to ischemia and reperfusion. Mitochondr Physiol Network 21.11
Moore 2016 Abstract IOC116	Moore AL (2016) Investigations into the multi-faceted roles of the alternative oxidases: are they a reflection of the nature of the hydrophobic cavity? Mitochondr Physiol Network 21.11
... further results

Team and guest tutors

Gnaiger Erich, CEO, OROBOROS INSTRUMENTS and Medical University of Innsbruck, D. Swarovski Research Laboratory

Doerrier Carolina, Chief Scientific Officer (CSO), Mitochondrial Physiology Specialist, OROBOROS INSTRUMENTS

Sumbalova Zuzana, Principal Investigator (PI), Mitochondrial Physiology Specialist, OROBOROS INSTRUMENTS

Chinopoulos Christos, Department of Medical Biochemistry, Semmelweis University, Budapest (HU)

Meszaros Andras, University of Szeged (HU)

Komlodi Timea: Department of Medical Biochemistry, Semmelweis University, Budapest (HU)

Sobotka Ondrej, Department of Physiology, Charles University in Prague, Hradec Kralove (CZ)

Participants

	Participant	Institution
	Aparicio Trejo Omar Emiliano	MX Mexico City Pedraza Chaverri J: Department of Biology, University of Mexico, Mexico City (MX)
	Bhuvanachandran Nair Syam	SE Gothenburg Hagberg H: Institute of Neuroscience and Physiology, University of Gothenburg (SE)
	Casado Pinna M	ES Valencia Casado Pinna M: Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia (ES)
	Cecatto Cristiane	BR Porto Alegre Souza DOG: Universidade Federal do Rio Grande do SuL, Porto Alegre(BR)
	Einer Claudia	DE Munich Zischka H: Helmholtz Centre Munich (DE)
	Grewal Rekha	DE Giessen Eckert GP: Department of Nutrition in Prevention & Therapy, Justus Liebig University, Giessen (DE)
	Goy Christine	DE Duesseldorf Haendeler J: Leibniz Research Institute for Environmental Medicine, Molecular Aging Research, Duesseldorf (DE)
	Hey-Mogensen Martin	US WA Seattle Hey-Mogensen M: Novo Nordisk Research Center, Seattle (US)
	Krizova Jana	CZ Prague Zeman J: Charles University and General University Hospital in Prague (CZ)
	Komlodi Timea	HU Budapest Tretter L: Department of Medical Biochemistry, Semmelweis University, Budapest (HU)
	Kraunsoee Regitze	DK Copenhagen Dela F: Department of Biomedical Sciences, University of Copenhagen (DK)
	Matsumoto Junichi	JP Sapporo Yokota T: Department of Cardiovascular Medicine, Hokkaido University, Sapporo (JP)
	Meszaros Andras	HU Szeged Boros M: University of Szeged (HU)
	Moore Anthony L	UK Brighton Moore AL: University of Sussex, Brighton (UK)
	Morato Fornaguera Laia	CH Lausanne Sandi C: EPFL, Lausanne (CH)
	Neves Pedro	AT Innsbruck OROBOROS: OROBOROS INSTRUMENTS (AT)
	Nielsen Birgitte	DK Copenhagen Poulsen HE: Rigshospitalet Copenhagen, Lab of clinical Pharamacology (DK)
	Poddar Jit	IN Haldia Chakrabarti S: ICARE Institute of Medical Sciences and Research, Haldia (IN)
	Schaefer Carmina	DE Giessen Eckert GP: Department of Nutrition in Prevention & Therapy, Justus Liebig University, Giessen (DE)
	Sigl Reinhard	AT Innsbruck OROBOROS: OROBOROS INSTRUMENTS (AT)
	Sobotka Ondrej	CZ Hradec Kralove Cervinkova Z: Department of Physiology, Charles University in Prague, Hradec Kralove (CZ)
	Spinazzi Marco	BE Leuven Spinazzi M: KU Leuven Center for Human, VIB Center for the Biology of Disease (BE)
	Weir Mary	CA Antigonish Kane DA: St. Francis Xavier University, Antigonish (CA)
	Wyss Rahel	CH Bern Longnus S: Bern University Hospital, Department of Cardiovascular Surgery (CH)

General information

This O2k-Workshop for advanced users provides a short introduction to HRR. The O2k-Fluo LED2-Module and O2k-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin, TMRM), ATP production (Mg green), and Ca²⁺ (Ca green) will be discussed.

» Information on travel and venue: IOC Innsbruck.

Venue

Hotel Grauer Bär

Universitätsstrasse 5-7, 6020 Innsbruck

Tel: +43 (0) 512 5924

Our prices include accommodation at Hotel Grauer Bär, full board, coffee breaks, spa area

Registration

Please fill in the registration form and send it to [email protected]

» Registration pdf

Support

IOC student scholarships are available for this IOC. More information? See here.

O2k perspectives - abstracts

Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.

» Abstract guidelines

IOC recommended reading

» IOC recommended reading

» MitoPedia: Respiratory states

Contact

[email protected]

OROBOROS INSTRUMENTS

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

O2k - tested and trusted

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.

O2k-Workshops are listed as MitoGlobal Events.

Labels:

ORO, IOC, 2016, MitoFit, Next

@@ Line 10: / Line 10: @@
 '''OROBOROS O2k-Workshop on O2k-Fluorometry - advanced'''. Innsbruck, Austria; 2016 Nov 21-25.
 |mipnetlab=AT_Innsbruck_OROBOROS
-}}
-{{Labeling
-|additional=ORO, IOC, 2016, MitoFit, Next
 }}
 __TOC__
@@ Line 43: / Line 40: @@
 |-
 | 08:30
-| [[Gnaiger E |Erich Gnaiger]]: Introduction O2k-Fluorometry: mt-membrane potential and H2O2 production.
+| [[File:Gnaiger Erich.jpg|50px|left|Erich Gnaiger]] [[Gnaiger E |Erich Gnaiger]]
+Introduction O2k-Fluorometry: mt-membrane potential and H2O2 production.
 |-
 | 09:30
-| [[Gnaiger E |Erich Gnaiger]]:  O2k-Fluo demo experiment: H2O2 production in permeabilized HEK cells.
+| [[Image:O2k-Fluorometer.JPG|50px|left|link=O2k-Fluorometer]] [[Doerrier C |Carolina Doerrier]], [[Gnaiger E |Erich Gnaiger]]
+O2k-Fluo demo experiment: H2O2 production in permeabilized HEK cells.
 |-
 | 10:30
@@ Line 52: / Line 53: @@
 |-
 | 10:45
-| [[Doerrier C |Carolina Doerrier]]: Hands-on experiment: Respiration and H2O2 production in permeabilized HEK cells.
+| [[File:DoerrierC.JPG|50px|left|Carolina Doerrier]] [[Doerrier C |Carolina Doerrier]]
+Hands-on experiment: Respiration and H2O2 production in permeabilized HEK cells.
 |-
 | 12:30
@@ Line 58: / Line 61: @@
 |-
 | 14:00
-| DatLab analysis and collection of experimental data.
+| [[Image:Logo OROBOROS-DatLab.jpg|50px|left|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]
+DatLab analysis and collection of experimental data.
 |-
 | 15:30
-| [[Moore_Anthony| Anthony Moore]]: Investigations into the multi-faceted roles of the alternative oxidases: are they a reflection of the nature of the hydrophobic cavity?
+| [[File:Moore Anthony L.jpg|50px|left|Anthony Moore]] [[Moore_AL| Anthony Moore]]
+Investigations into the multi-faceted roles of the alternative oxidases: are they a reflection of the nature of the hydrophobic cavity?
 |-
 | 16:00
@@ Line 67: / Line 74: @@
 |-
 | 16:15
-| [[Gnaiger E |Erich Gnaiger]]: Data discussion, SUIT protocol interpretation, summary.
+| [[Image:O2k-Network.png|left|50px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] OROBOROS-Team and Guest Tutors
+Data discussion, SUIT protocol interpretation, summary.
 |-
 | 18:00
@@ Line 85: / Line 94: @@
 |-
 | 08:30
-| [[Sumbalova Z |Zuzana Sumbalova]]: O2k-potentiometric measurement of mt-membrane potential with TPP+: TPP+ calibration and demo with mouse brain isolated mitochondria.
+| [[File:SumbalovaZ.JPG|left|50px|Zuzana Sumbalova]] [[Sumbalova Z |Zuzana Sumbalova]]
+O2k-potentiometric measurement of mt-membrane potential with TPP+: TPP+ calibration and demo with mouse brain isolated mitochondria.
 |-
 | 10:30
@@ Line 91: / Line 102: @@
 |-
 | 10:45
-| Demo with mouse brain isolated mitochondria.
+| [[File:O2k-TPP-ISE.jpg|left|50px|link=http://wiki.oroboros.at/index.php/O2k-TPP%2B_ISE-Module|TPP-ISE]]
+Demo with mouse brain isolated mitochondria.
 |-
 | 12:30
@@ Line 97: / Line 110: @@
 |-
 | 14:00
-|Hands-on: TPP+ or O2k-Fluorometry: Safranin or TMRM.
+| [[Image:O2k-Fluorometer.JPG|50px|left|link=O2k-Fluorometer]]
+Hands-on: TPP+ or O2k-Fluorometry: Safranin or TMRM.
 |-
 | 16:00
@@ Line 103: / Line 118: @@
 |-
 | 16:15
-|[[Sumbalova Z |Zuzana Sumbalova]]: Data analysis and discussion.
+| [[Image:Logo OROBOROS-DatLab.jpg|50px|left|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[Sumbalova Z |Zuzana Sumbalova]]
+Data analysis and discussion.
 |-
 | 18:00
@@ Line 121: / Line 138: @@
 |-
 | 08:30
-| [[Chinopoulos C |Chinopoulos Christos]]: Determination of ADP-ATP exchange rate in isolated mitochondria.
+| [[Image:Christos Chinopoulos.jpg|50px|left|Christos Chinopoulos]] [[Chinopoulos C |Christos Chinopoulos]]
+Determination of ADP-ATP exchange rate in isolated mitochondria.
 |-
 | 10:30
@@ Line 127: / Line 146: @@
 |-
 | 10:45
-| [[Chinopoulos C |Chinopoulos Christos]]: Demo experiment: O2k-Fluo measurement (Mg-Green) of the ~P/O2 ratio in isolated mitochondria.
+| [[File:Fluorescence-Sensor Green.JPG|left|50px]] [[Chinopoulos C |Christos Chinopoulos]]
+Demo experiment: O2k-Fluo measurement (Mg-Green) of the ~P/O2 ratio in isolated mitochondria.
 |-
 | 12:30
@@ Line 133: / Line 154: @@
 |-
 | 14:00
-| [[Chinopoulos C |Chinopoulos Christos]]: Data analysis and summary of experimental results.
+| [[Image:O2k-Fluorometer.JPG|50px|left|link=O2k-Fluorometer]] [[Chinopoulos C |Christos Chinopoulos]]
+Data analysis and summary of experimental results.
 |-
 | 16:00
@@ Line 139: / Line 162: @@
 |-
 | 16:30
-| ''Glühwein at the Christkindl market'' in the Old Town.
+| [[File:Christkindlmarkt Innsbruck.jpg|50px]] ''Glühwein at the Christkindl market'' in the Old Town.
 |-
 | 18:00
@@ Line 149: / Line 172: @@
 |}
+=== Abstracts ===
-== General information ==
+{{#ask:[[was submitted to event::IOC116]]
-::::This O2k-Workshop for advanced users provides a short introduction to HRR. The O2k-Fluo LED2-Module and O2k-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin, TMRM), ATP production (Mg green), and Ca<sup>2+</sup> (Ca green) will be discussed.
+|?has title
-::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
+|limit=30
+|format=table
-== Registration ==
+|sort=was written by
-:::: Please fill in the registration form and send it to [mailto:[email protected]| [email protected]]
+|order=ascending
-::::» [[Media:Registration Form IOC116 2016-11 Innsbruck.pdf| Registration pdf]]
+|default=no abstracts found<br /><br />
+}}
-== Support ==
-:::: IOC student scholarships are available for this IOC. More information? [[IOC_student_scholarship|See here]].
@@ Line 244: / Line 265: @@
 | '''[[HU Szeged Boros M]]:'''  University of Szeged (HU)
 |-
-|
+| [[File:Moore Anthony L.jpg|90px|Anthony Moore]]
-| [[Moore Anthony]]
+| [[Moore AL|Moore Anthony L]]
 | '''UK Brighton Moore AL:''' University of Sussex, Brighton (UK)
 |-
@@ Line 252: / Line 273: @@
 | '''[[CH Lausanne Sandi C]]:''' EPFL, Lausanne (CH)
 |-
-|
+| [[File:NevesP.JPG|90px|Pedro Neves]]
 | [[Neves Pedro]]
 | '''[[AT Innsbruck OROBOROS]]:''' OROBOROS INSTRUMENTS (AT)
@@ Line 289: / Line 310: @@
 |}
-== O2k perspectives - abstracts ==
+== General information ==
+:::: This O2k-Workshop for advanced users provides a short introduction to HRR. The O2k-Fluo LED2-Module and O2k-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin, TMRM), ATP production (Mg green), and Ca<sup>2+</sup> (Ca green) will be discussed.
+::::» Information on travel and venue: '''[[IOC Innsbruck]]'''.
+::: '''Venue'''
+:::: [http://www.grauer-baer.at Hotel Grauer Bär]
+:::: Universitätsstrasse 5-7, 6020 Innsbruck
+:::: Tel: +43 (0) 512 5924
+:::: Our prices include accommodation at Hotel Grauer Bär, full board, coffee breaks, spa area
+::: '''Registration'''
+:::: Please fill in the registration form and send it to [mailto:[email protected]| [email protected]]
+::::» [[Media:Registration Form IOC116 2016-11 Innsbruck.pdf| Registration pdf]]
+::: '''Support'''
+:::: IOC student scholarships are available for this IOC. More information? [[IOC_student_scholarship|See here]].
+::: '''O2k perspectives - abstracts'''
 :::: Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.
 ::::» [[Abstracts_IOC_help#Abstracts |'''Abstract guidelines''']]
-{{#ask:[[was submitted to event::IOC116]]
-|?has title
-|limit=30
-|format=table
-|sort=was written by
-|order=ascending
-|default=no abstracts found<br /><br />
-}}
-== Venue ==
+::: '''IOC recommended reading'''
-::::[http://www.grauer-baer.at Hotel Grauer Bär]
+::::» '''[[IOC recommended reading]]'''
-::::Universitätsstrasse 5-7, 6020 Innsbruck
+::::» '''[[MitoPedia: Respiratory states]]'''
-::::Tel: +43 (0) 512 5924
+:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
-::::Our prices include accommodation at Hotel Grauer Bär, full board, coffee breaks, spa area
-== Contact ==
+::: '''Contact'''
 :::: [mailto:[email protected]| [email protected]]
 :::: OROBOROS INSTRUMENTS
 :::: high-resolution respirometry
@@ Line 321: / Line 348: @@
 :::: '''O2k - tested and trusted'''
-== Project support==
+::: '''Project support'''
 :::: Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
 :::: [[File:Sponsors.png| 750 px]]
-== IOC recommended reading ==
-::::» '''[[IOC recommended reading]]'''
-::::» '''[[MitoPedia: Respiratory states]]'''
-:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
@@ Line 336: / Line 357: @@
+{{Labeling
+|additional=ORO, IOC, 2016, MitoFit, Next
+}}
 [[Category:O2k-Workshops]]