Difference between revisions of "MiPNet21.06 SUIT RP"

Revision as of 18:58, 19 January 2016

SUIT reference assay for OXPHOS analysis by high-resolution respirometry. - »Bioblast pdf«

» Versions

OROBOROS (2016-01-19) Mitochondr Physiol Network

Abstract: Doerrier C, Sumbalova Z, Lamberti G, Krumschnabel G, Gnaiger (2016) SUIT reference assay for OXPHOS analysis by high-resolution respirometry. Mitochondr Physiol Network 21.06(pre03):1-10.

» Instrument: O2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria

Regulation: Substrate Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

O2k-SOP

Laboratory sheets for SUIT protocols RP1 and RP2

SUIT reference assay for permeabilized fibres, Pfi

Type	SUIT protocol	Sample	Abbr	pdf file	docx file
Titration sheet	RP1	Permeabilized fibres	Pfi	»SUIT_RP1-Pfi	»SUIT_RP1-Pfi
Titration sheet	RP2		Pfi	»SUIT_RP2-Pfi	»SUIT_RP2-Pfi
Tick boxes	Combined		Pfi	»RP print-Pfi	»RP print-Pfi
Titration sheet	RP1	Permeabilized cells	Pc	»SUIT_RP1	»SUIT_RP1
Titration sheet	RP2		Pc	»SUIT_RP2	»SUIT_RP2
Tick boxes	Combined		Pc	»RP-Pc print	»RP-Pc print
Titration sheet	RP1	Isolated mitochondria, tissue homogenate	Imt, Thom	»SUIT_RP1	»SUIT_RP1
Titration sheet	RP2		Imt, Thom	»SUIT_RP2	»SUIT_RP2
Tick boxes	Combined		Imt, Thom	»RP print	»RP print

Experimental details

Saturating ADP concentrations

Different concentrations of ADP are sufficient to obtain maximum flux for estimating OXPHOS capacity. The typical range for isolated mitochondria is 1 to 2.5 mM, for permeabilized cells 1 to 5 mM, for permeabilized muscle fibres 2.5 to 10 mM.

Pre-publication communication

This communication is a pre-publication, inviting critical feedback and comments. All feedback will be carefully documented and evaluated in terms of justification of co-authorship. We intend to finally publish this topic in a peer-reviewed journal as an original article, with reference to the pre-publication history including reviewer's reports. - Gnaiger Erich 03:23, 18 January 2016 (CET)

Further details

Introduction » Gnaiger 2014 MitoPathways
Table of titrations » MiPNet09.12 O2k-Titrations
Definition » Substrate-uncoupler-inhibitor titration
Context » SUIT protocol library
Abbreviations » MitoPedia

@@ Line 28: / Line 28: @@
 ! SUIT protocol
 ! Sample
+! Abbr
 ! pdf file
 ! docx file
@@ Line 33: / Line 34: @@
 | Titration sheet
 | RP1
+| Permeabilized fibres
 | Pfi
 | [[Media:SUIT_RP1-Pfi.pdf |»SUIT_RP1-Pfi]]
@@ Line 39: / Line 41: @@
 | Titration sheet
 | RP2
+|
 | Pfi
 | [[Media:SUIT_RP2-Pfi.pdf |»SUIT_RP2-Pfi]]
@@ Line 45: / Line 48: @@
 | Tick boxes
 | Combined
+|
 | Pfi
 | [[Media:RP-Pfi print.pdf |»RP print-Pfi]]
 | [[Media:RP-Pfi print.docx |»RP print-Pfi]]
+|-|-
+| Titration sheet
+| RP1
+| Permeabilized cells
+| Pc
+| [[Media:SUIT_RP1-Pc.pdf |»SUIT_RP1]]
+| [[Media:SUIT_RP1-Pc.docx |»SUIT_RP1]]
+|-
+| Titration sheet
+| RP2
+|
+| Pc
+| [[Media:SUIT_RP2-Pc.pdf |»SUIT_RP2]]
+| [[Media:SUIT_RP2-Pc.docx |»SUIT_RP2]]
+|-
+| Tick boxes
+| Combined
+|
+| Pc
+| [[Media:RP print.pdf |»RP-Pc print]]
+| [[Media:RP print.docx |»RP-Pc print]]
 |-
 | Titration sheet
 | RP1
-| mt
+| Isolated mitochondria, tissue homogenate
+| Imt, Thom
 | [[Media:SUIT_RP1.pdf |»SUIT_RP1]]
 | [[Media:SUIT_RP1.docx |»SUIT_RP1]]
@@ Line 57: / Line 83: @@
 | Titration sheet
 | RP2
-| mt
+|
+| Imt, Thom
 | [[Media:SUIT_RP2.pdf |»SUIT_RP2]]
 | [[Media:SUIT_RP2.docx |»SUIT_RP2]]
@@ Line 63: / Line 90: @@
 | Tick boxes
 | Combined
-| mt
+|
+| Imt, Thom
 | [[Media:RP print.pdf |»RP print]]
 | [[Media:RP print.docx |»RP print]]
 |-
 |}
 == Experimental details ==