MiPNet19.18E O2 flux analysis - DatLab 6: Difference between revisions
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Revision as of 14:27, 20 October 2010
Gnaiger E. Oxygen flux analysis: On-line DatLab4.3. Mitochondr. Physiol. Network 12.09. |
ยป MiPNet12.09
) Mitochondr. Physiol. Network
Abstract: DatLab 4 sets a novel standard in high-resolution respirometry, for on-line analysis of oxygen flux measured in the OROBOROS Oxygraph-2k, and of other signals obtained in the O2k- MultiSensor System. Oxygen flux can be plotted with or without O2kbackground correction, is instantaneously expressed per mass of sample or per number of cells. Various sections on the plot of oxygen flux are marked, and corresponding average values are viewed in a table which can be simply exported into Excel or SigmaPlot. Instrumental and experimental parameters are summarized in a protocol, which can be printed or saved as a pdf file. These new features provide the basis for combining high-resolution with instant and user-friendly analysis. A demonstration experiment, performed during an Oxygraph-2k Workshop on high-resolution respirometry, is used as an example for application of DatLab 4 and DatLab-Excel templates MiPNet08.09. All analyses can be performed on-line or off-line. โข Keywords: HRR-DatLab
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k, DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Info
MiPNet10.01 is integrated in MiPNet12.09_O2FluxAnalysis.