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MiPNet19.18B POS-service

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MiPNet19.18B POS-service

Publications in the MiPMap
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.

» Bioblast pdf »Versions

Oroboros (2016-08-08) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) Service of the polarographic oxygen sensor OroboPOS. Mitochondr Physiol Network 19.18(B04):17-22. »

» O2k-Videosupport
» O2k-SOP: Polarographic oxygen sensors: calibration, accuracy and quality control SOP. »POS-calibration-SOP«
» Product: OroboPOS

Keywords: HRR

O2k-Network Lab: AT_Innsbruck_Oroboros


Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, O2k-Manual 

O2k-Core, DL6, DL7, DL6a7, SeriesG, SeriesH 



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



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Anode treatment by ammonia

The anode of the POS consists of a solid silver ring coated with silver/silver chloride. The AgCl coating will grow during use. A typical contamination is the formation of AgO. AgO is removed by the ammonia treatment during sensor service, however part of the AgCl layer is removed in the process. Defects in the AgCl layer are restored by running the O2k for a few hours (or over night) after sensor service and by continued use.


Delayed and slow response of the sensor

A slow POS has a long response time, responding to a change of oxygen partial pressure very slowly. The sensor response time can be most conveniently checked by the stirrer test.
A slow oxygen sensor cannot be used for kinetic studies, and may require prolonged periods of measurement at steady states. Individual POS have different response times, which change after sensor service. A slow response can be improved by:
  1. Sensor service and application of a new membrane. After sensor service, the POS needs some time in operation to stabilize, which may be a few or several hours (overnight). During this stabilization time the oxygraph has to be running. After a sensor has been used and the seal tip has been removed from the sensor it is normal to see many small bubbles. This does not indicate that there was a problem while the sensor was actually in use.
  2. Theoretically a prolonged response time of a POS could be due to a hardware problem, which cannot be solved by intensive sensor service repeated several times. You may choose to ship the defective POS for special service to our workshop. We cannot predict, however, if such a special service will be successful.
  3. If not successful, the POS has to be replaced.


Sensor service by OROBOROS

We offer a sensor service in our workshop. This is essentially the same that you can do on your own. The main advantage of the sensor service in our workshop is the presence of a very experienced and very patient team, carrying out many iterations of NH3 cleaning. Running the O2k over night may be required to stabilize a newly serviced OroboPOS.


References