MiPNet19.09 IOC94 Shanghai CN: Difference between revisions

From Bioblast
No edit summary
No edit summary
 
(14 intermediate revisions by 3 users not shown)
Line 2: Line 2:
{{Publication
{{Publication
|title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]  
|title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]  
'''Shanghai CN''', 2014 Jun 27-Jul 02. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC94'''. OROBOROS representative in China: »[[CN Beijing Zenda]].  
'''Shanghai CN''', 2014 Jun 27-Jul 02. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, '''IOC94'''. Oroboros representative in China: »[[CN Shanghai Zenda]].
|info=[[File:PDF.jpg|100px|link= http://wiki.oroboros.at/images/7/7a/MiPNet19.09_IOC94_2014-06_Shanghai.pdf|Bioblast pdf]]  
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/7/7a/MiPNet19.09_IOC94_2014-06_Shanghai.pdf|Bioblast pdf]]
|authors=OROBOROS
|authors=Oroboros
|year=2014-06-27
|year=2014-06-27
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
'''94th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' in cooperation with China Representative '''[[CN Beijing Zenda]]'''. [http://wiki.oroboros.at/index.php/File:MiPNet19.09_IOC94_2014-06_Shanghai.pdf Versions]
'''94th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' in cooperation with China Representative '''[[CN Shanghai Zenda]]'''. [http://wiki.oroboros.at/index.php/File:MiPNet19.09_IOC94_2014-06_Shanghai.pdf Versions]
  Experimental results from O2k-Demo experiments: [[Media:MiPNet19.09 IOC94 2014-06 Shanghai.pdf |»Bioblast pdf«]]
  Experimental results from O2k-Demo experiments: [[Media:MiPNet19.09 IOC94 2014-06 Shanghai.pdf |»Bioblast pdf«]]
|mipnetlab=CN Beijing Zenda, AT Innsbruck OROBOROS
|mipnetlab=CN Shanghai Zenda, AT Innsbruck Oroboros
}}
}}
{{Labeling
|area=Respiration
|organism=Saccharomyces cerevisiae
|taxonomic group=Hexapods
|preparations=Intact cells
|injuries=Ischemia-reperfusion
|topics=Oxygen kinetics
|couplingstates=ROUTINE
|instruments=Oxygraph-2k, O2k-Fluorometer
|additional=ORO, IOC, 2014, O2k-Network, MitoCom
}}


'''»''' [[MitoPedia: Respiratory states]]
'''»''' [[MitoPedia: Respiratory states]]
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]


[[File:Erich Gnaiger-IOC 2431.JPG|right|300px|Erich Gnaiger]]
[[File:Erich Gnaiger-IOC 2431.JPG|right|300px|Erich Gnaiger]]
Line 75: Line 60:


=== Venue ===
=== Venue ===
:: [[CN Beijing Zenda]]
:: [[CN Shanghai Zenda]]
:: Room 105, Building 3, Block 1, Mingpu Plaza
:: Room 105, Building 3, Block 1, Mingpu Plaza
:: 3279 Sanlu Road, Minhang District, Shanghai, China
:: 3279 Sanlu Road, Minhang District, Shanghai, China
Line 83: Line 68:
=== Registration and general information ===
=== Registration and general information ===
[[Image:Logo OROBOROS INSTRUMENTS.jpg|left|40px|link=http://www.oroboros.at|OROBOROS]]
[[Image:Logo OROBOROS INSTRUMENTS.jpg|left|40px|link=http://www.oroboros.at|OROBOROS]]
: » [[CN Beijing Zenda]]
: » [[CN Shanghai Zenda]]


== OROBOROS O2k-Network Reference Labs in China ==
== OROBOROS O2k-Network Reference Labs in China ==
Line 92: Line 77:
<big>'''Key references'''</big>
<big>'''Key references'''</big>
[[Image:O2k-Publications.jpg|left|50px|link=http://www.bioblast.at/index.php/O2k-Publications: Topics|O2k-Publications in the MiPMap]]
[[Image:O2k-Publications.jpg|left|50px|link=http://www.bioblast.at/index.php/O2k-Publications: Topics|O2k-Publications in the MiPMap]]
:* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]]
:* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]]
:* Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053. [[Perry 2013 Diabetes |»PMID: 23520284]]
:* Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53. [[Perry 2013 Diabetes |»PMID: 23520284]]
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]




<big>'''Introduction and O2k-Core Manual'''</big>
<big>'''Introduction and O2k-Core Manual'''</big>
[[Image:O2k-Manual.jpg|left|30px|link=http://oroboros.at/?O2k-Manual|O2k-Manual]]
[[Image:O2k-Manual.jpg|left|30px|link=http://oroboros.at/?O2k-Manual|O2k-Manual]]
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [[Gnaiger_2008_POS |»Bioblast Access]]
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]]
* [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]
* [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]


Line 105: Line 90:
<big>'''HRR and O2k-Fluorometry'''</big>
<big>'''HRR and O2k-Fluorometry'''</big>
* »[[MiPNet17.05 O2k-Fluo LED2-Module|Manual: O2k-Fluo LED2-Module]]
* »[[MiPNet17.05 O2k-Fluo LED2-Module|Manual: O2k-Fluo LED2-Module]]
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''


Line 126: Line 111:
  O2k-Workshops are listed as [[MitoGlobal Events]].
  O2k-Workshops are listed as [[MitoGlobal Events]].
* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''.
* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''.
{{Labeling
|preparations=Intact cells
|instruments=Oxygraph-2k
|additional=ORO, IOC, 2014, O2k-Network, MitoCom, CN
}}


[[Category:O2k-Workshops]]
[[Category:O2k-Workshops]]

Latest revision as of 15:12, 8 June 2020

                



MiPNet19.09 IOC94 Shanghai CN

Publications in the MiPMap
O2k-Network

Shanghai CN, 2014 Jun 27-Jul 02. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC94. Oroboros representative in China: »CN Shanghai Zenda.

» Bioblast pdf

Oroboros (2014-06-27) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

94th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry in cooperation with China Representative CN Shanghai Zenda. Versions

Experimental results from O2k-Demo experiments: »Bioblast pdf«


O2k-Network Lab: CN Shanghai Zenda, AT Innsbruck Oroboros


» MitoPedia: Respiratory states OXPHOS ETS ROUTINE LEAK ROX

Erich Gnaiger

Lecturer

Erich Gnaiger

Gnaiger Erich, CEO, OROBOROS INSTRUMENTS

Keynote at conference:

Life Style and Mitochondrial Competence – modern drugs for T2 Diabetes in aging and degenerative diseases.



  • Ming Cheng, ZENDA LLC, Sales manager, Wuhan Office. Tel: 86-13554361876
  • Xin Yuan, ZENDA LLC, Shanghai office. Tel: 86-15821657315, [email protected]
  • Meijan Chai, ZENDA LLC, Shanghai office. Tel: 86-18501643129, [email protected]
  • Fangyi Du, ZENDA LLC, Beijing Office. Tel: 86-13641079436
  • Huixing Xin, ZENDA LLC, Beijing Office. Tel: 86-19610025801

Programme

Date Event Comment
2014-06-27 to 28 E. Gnaiger (keynote; June 28th); Zenda LLC & OROBOROS INSTRUMENTS (booth) at:

2014 Workshop of Translational Diabetes & Metabolic Disease Medicine

Programme: http://www.bioon.com/z/2014dm/agenda.shtml

http://www.bioon.com/z/2014dm/
2014-06-30 O2k-Seminar: General introduction for scientists and clinicians interested in mitochondrial physiology and pathology. Zenda, Mingpu Plaza
2014-07-01 to 02 O2k-Workshop: Practical introduction for O2k-Users. Zenda, Mingpu Plaza (limited number of participants)

Venue

CN Shanghai Zenda
Room 105, Building 3, Block 1, Mingpu Plaza
3279 Sanlu Road, Minhang District, Shanghai, China
  • Due to the overwhelming response from our customers, we probably will have over 40 people attending the seminar in Shanghai (Jintao Zeng, Zenda LLC).

Registration and general information

OROBOROS
» CN Shanghai Zenda

OROBOROS O2k-Network Reference Labs in China

» O2k-Network Labs China

Recommended reading

Key references

O2k-Publications in the MiPMap
  • Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Pubmed 24862266
  • Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53. »PMID: 23520284
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access


Introduction and O2k-Core Manual

O2k-Manual
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
  • »O2k-Core Manual.pdf


HRR and O2k-Fluorometry


Mitochondrial pathways

O2k-Protocols

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.


Diabetes and metabolic diseases



MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.
  • This workshop is a component of the K-Regio project MitoCom.


Labels:



Preparation: Intact cells 



HRR: Oxygraph-2k 

ORO, IOC, 2014, O2k-Network, MitoCom, CN 

Cookies help us deliver our services. By using our services, you agree to our use of cookies.