Difference between revisions of "MiPNet18.11 IOC82"
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{{Publication | {{Publication | ||
|title= | |title=82nd Workshop on high-resolution respirometry & O2k-Fluorometry. 2013 Dec 10-11. Trondheim, Norway. Satellite to the [www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway]. 2013 Nov 11-13. Mitochondr Physiol Network 18.11. | ||
|info=[[Media:MiPNet18.11 IOC82 2013-12 Trondheim.pdf|'''MiPNet18.11.pdf''']] [[Image:O2k-Workshops.png|right|150px|O2k-Workshops|link=http://www.oroboros.at/?o2k-workshops]] | |info=[[Media:MiPNet18.11 IOC82 2013-12 Trondheim.pdf|'''MiPNet18.11.pdf''']] [[Image:O2k-Workshops.png|right|150px|O2k-Workshops|link=http://www.oroboros.at/?o2k-workshops]] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2013 | |year=2013 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=O2k-Workshop on | |abstract=The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the OROBOROS Oxygraph-2k with integrated on-line analysis, introducing new features of DatLab 5.2. | ||
:>> | |||
The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer system and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology. | |||
The [[NO_Trondheim_Rognmo_O|Cardiac Exercise Research Group (Norwegian University of Science and Technology, Trondheim)]] is a MiPNet Lab offering their facility and O2ks for the training at the O2k-Workshop. The MiPNet Lab [[NO_Trondheim_Rognmo_O]] uses three O2k-instruments as a [http://www.oroboros.at/?Power-O2k Power-O2k Laboratory]. | |||
:>> [http://oroboros.at/?O2k-Workshops O2k-Workshop: current dates] | |||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom | |mipnetlab=AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom | ||
|titel=IOC82. Workshop on high-resolution respirometry. Trondheim, NO 09-11 December 2013. Mitochondr Physiol Network 18.11. | |titel=IOC82. Workshop on high-resolution respirometry. Trondheim, NO 09-11 December 2013. Mitochondr Physiol Network 18.11. | ||
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{{Labeling | {{Labeling | ||
|additional=IOC | |additional=IOC | ||
|titel=IOC82. Workshop on high-resolution respirometry. Trondheim, NO 09-11 December 2013. Mitochondr Physiol Network 18.11. | |||
|link= | |||
|editor= | |||
|high-resolution_respirometry= | |||
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}} | }} | ||
__NOTOC__ | __NOTOC__ | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] | ||
Revision as of 15:51, 13 December 2013
| 82nd Workshop on high-resolution respirometry & O2k-Fluorometry. 2013 Dec 10-11. Trondheim, Norway. Satellite to the [www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway]. 2013 Nov 11-13. Mitochondr Physiol Network 18.11. |
OROBOROS (2013) Mitochondr Physiol Network
Abstract: The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the OROBOROS Oxygraph-2k with integrated on-line analysis, introducing new features of DatLab 5.2.
The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer system and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology.
The Cardiac Exercise Research Group (Norwegian University of Science and Technology, Trondheim) is a MiPNet Lab offering their facility and O2ks for the training at the O2k-Workshop. The MiPNet Lab NO_Trondheim_Rognmo_O uses three O2k-instruments as a Power-O2k Laboratory.
- >> O2k-Workshop: current dates
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom
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