Difference between revisions of "MiPNet18.11 IOC82"
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{{Publication | {{Publication | ||
|title= | |title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]] | ||
|info= [[ | '''Trondheim NO''', 2013 Dec 10-11, Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, Oroboros '''IOC82'''. Satellite to the [http://www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway], O2k-Network Lab »[[NO Trondheim Rognmo O]]. | ||
|authors= | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/0/0e/MiPNet18.11_IOC82_2013-12_Trondheim.pdf |Bioblast pdf]] | ||
|year=2013 | |authors=Oroboros | ||
|year=2013-12-10 | |||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=O2k- | |abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]] | ||
[[Image:IOC82_Trondheim_2013-12-13.JPG|400px|right|link=http://www.bioblast.at/index.php/NO_Trondheim_Rognmo_O|MiPNet Lab NO_Trondheim_Rognmo O]] | |||
: | '''The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the Oroboros O2k with integrated on-line analysis, introducing new features of DatLab 5.2.''' »[http://www.bioblast.at/index.php/File:MiPNet18.11_IOC82_2013-12_Trondheim.pdf Versions] | ||
|mipnetlab= | The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer-pathway and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology. | ||
The [[NO_Trondheim_Rognmo_O|Cardiac Exercise Research Group (Norwegian University of Science and Technology, Trondheim)]] is a MiPNet Lab offering their facility and O2ks for the training at the O2k-Workshop. The MiPNet Lab [[NO_Trondheim_Rognmo_O]] uses three O2k-instruments as a [[Power-O2k| Power-O2k Laboratory]]. | |||
:>> [http://www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway] | |||
|mipnetlab=AT_Innsbruck_Oroboros , AT Innsbruck MitoCom, NO Trondheim Rognmo O | |||
|titel=IOC82. Workshop on high-resolution respirometry. Trondheim, NO 09-11 December 2013. Mitochondr Physiol Network 18.11. | |titel=IOC82. Workshop on high-resolution respirometry. Trondheim, NO 09-11 December 2013. Mitochondr Physiol Network 18.11. | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|additional=IOC | |instruments=Oxygraph-2k | ||
|additional=ORO, IOC, 2013, O2k-Network | |||
}} | }} | ||
[[File:IOC82 Trondheim Christopher McDougall, Verena Laner.JPG|150px|right|Christopher McDougall, Verena Laner]] | |||
== Special evening: Man in Extreme Environments == | |||
Summary of the ''Man in Extreme Environments''-Evening: [[2013_Man_in_Extreme_Environments| Christopher McDougall - ''Born to Run'']] | |||
* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''. | |||
Latest revision as of 12:19, 23 January 2019
MiPNet18.11 IOC82
 Trondheim NO, 2013 Dec 10-11, Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, Oroboros IOC82. Satellite to the 5th Seminar on Exercise in Medicine. Trondheim, Norway, O2k-Network Lab »NO Trondheim Rognmo O. |
» 
Oroboros (2013-12-10) Mitochondr Physiol Network
Abstract:
The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the Oroboros O2k with integrated on-line analysis, introducing new features of DatLab 5.2. »Versions
The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer-pathway and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology.
The Cardiac Exercise Research Group (Norwegian University of Science and Technology, Trondheim) is a MiPNet Lab offering their facility and O2ks for the training at the O2k-Workshop. The MiPNet Lab NO_Trondheim_Rognmo_O uses three O2k-instruments as a Power-O2k Laboratory.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT Innsbruck MitoCom, NO Trondheim Rognmo O
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2013, O2k-Network
Special evening: Man in Extreme Environments
Summary of the Man in Extreme Environments-Evening: Christopher McDougall - Born to Run
- This workshop is a component of the K-Regio project MitoCom.
