Difference between revisions of "MiPNet18.11 IOC82"

Latest revision as of 12:19, 23 January 2019

MiPNet18.11 IOC82

Trondheim NO, 2013 Dec 10-11, Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, Oroboros IOC82. Satellite to the 5th Seminar on Exercise in Medicine. Trondheim, Norway, O2k-Network Lab »NO Trondheim Rognmo O.

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Oroboros (2013-12-10) Mitochondr Physiol Network

Abstract:

The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the Oroboros O2k with integrated on-line analysis, introducing new features of DatLab 5.2. »Versions

The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer-pathway and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology.

The Cardiac Exercise Research Group (Norwegian University of Science and Technology, Trondheim) is a MiPNet Lab offering their facility and O2ks for the training at the O2k-Workshop. The MiPNet Lab NO_Trondheim_Rognmo_O uses three O2k-instruments as a Power-O2k Laboratory.

>> 5th Seminar on Exercise in Medicine. Trondheim, Norway

• O2k-Network Lab: AT_Innsbruck_Oroboros, AT Innsbruck MitoCom, NO Trondheim Rognmo O

Labels:

HRR: Oxygraph-2k

ORO, IOC, 2013, O2k-Network

Special evening: Man in Extreme Environments

Summary of the Man in Extreme Environments-Evening: Christopher McDougall - Born to Run

This workshop is a component of the K-Regio project MitoCom.

@@ Line 2: / Line 2: @@
 {{Publication
 |title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]
-'''Trondheim NO''', 2013 Dec 10-11, OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, OROBOROS '''IOC82'''. Satellite to the [http://www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway], O2k-Network Lab »[[NO Trondheim Rognmo O]].
+'''Trondheim NO''', 2013 Dec 10-11, Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, Oroboros '''IOC82'''. Satellite to the [http://www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway], O2k-Network Lab »[[NO Trondheim Rognmo O]].
 |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/0/0e/MiPNet18.11_IOC82_2013-12_Trondheim.pdf |Bioblast pdf]]
-|authors=OROBOROS
+|authors=Oroboros
 |year=2013-12-10
 |journal=Mitochondr Physiol Network
 |abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
 [[Image:IOC82_Trondheim_2013-12-13.JPG|400px|right|link=http://www.bioblast.at/index.php/NO_Trondheim_Rognmo_O|MiPNet Lab NO_Trondheim_Rognmo O]]
-'''The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the OROBOROS O2k with integrated on-line analysis, introducing new features of DatLab 5.2.''' »[http://www.bioblast.at/index.php/File:MiPNet18.11_IOC82_2013-12_Trondheim.pdf Versions]
+'''The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the Oroboros O2k with integrated on-line analysis, introducing new features of DatLab 5.2.''' »[http://www.bioblast.at/index.php/File:MiPNet18.11_IOC82_2013-12_Trondheim.pdf Versions]
 The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer-pathway and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology.
@@ Line 16: / Line 16: @@
 :>> [http://www.ntnu.edu/cerg/seminar-on-exercise-in-medicine 5th Seminar on Exercise in Medicine. Trondheim, Norway]
-|mipnetlab=AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom, NO Trondheim Rognmo O
+|mipnetlab=AT_Innsbruck_Oroboros , AT Innsbruck MitoCom, NO Trondheim Rognmo O
 |titel=IOC82. Workshop on high-resolution respirometry. Trondheim, NO 09-11 December 2013. Mitochondr Physiol Network 18.11.
 }}