MiPNet18.05 Amplex-Mouse-heart

Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05.

» Bioblast Access - File:MiPNet18.05 Amplex-Mouse-heart.pdf

OROBOROS (2013) Mitochondr Physiol Network

Abstract: The O2k-Fluorescence LED2-Module is an extension of the OROBOROS Oxygraph-2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H₂O₂ production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H₂O₂ production.

Labels: MiParea: Respiration

Organism: Saccharomyces cerevisiae

Preparation: Intact cells

Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Uncoupler Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

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