MiPNet18.05 Amplex-Mouse-heart
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Oroboros (2019-06-24) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6.
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- » Product: Oroboros O2k, O2k-Catalogue
- » Permeabilized muscle fibers
• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom
