MiPNet18.05 Amplex-Mouse-heart
{{Publication
|title=
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
|info=
|authors=Oroboros
|year=2015-06-16
|journal=Mitochondr Physiol Network
|abstract=Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6. »Versions
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- >> Product: [[Oroboros O2k], O2k-Catalogue
- >> Permeabilized muscle fibres
|mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom |link= }}
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder
