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Difference between revisions of "MiPNet18.05 Amplex-Mouse-heart"

From Bioblast
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{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRFR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate.
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRFR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate.
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions]
|authors=Oroboros
|authors=Oroboros
|year=2015-06-16
|year=2015-06-16
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRFR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.''' »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions]
|abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRFR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.'''  


The [[O2k-Fluorescence LED2-Module]] is an extension of the Oroboros O2k for combining High-Resolution FluoRespirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production.
The [[O2k-Fluorescence LED2-Module]] is an extension of the Oroboros O2k for combining High-Resolution FluoRespirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production.

Revision as of 09:05, 11 January 2018

Publications in the MiPMap
O2k-Protocols
O2k-Fluorometry: HRFR and H2O2 production in mouse cardiac tissue homogenate.

» Bioblast pdf »Versions

Oroboros (2015-06-16) Mitochondr Physiol Network

Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRFR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.

The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining High-Resolution FluoRespirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.

>> Product: Oroboros O2k, O2k-Catalogue
>> Permeabilized muscle fibres


O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom


Labels: MiParea: Respiration 


Organism: Mouse  Tissue;cell: Heart  Preparation: Homogenate 

Regulation: Cyt c  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS  HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol 

O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder