Difference between revisions of "MiPNet18.05 Amplex-Mouse-heart"
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http:// | |title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. | ||
|info=[[Media:MiPNet18.05_Amplex-Mouse-heart.pdf|'''Open Access-MiPNet18.05.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions] | |info=[[Media:MiPNet18.05_Amplex-Mouse-heart.pdf|'''Open Access-MiPNet18.05.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2013-09 | |year=2013-09 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''O2k- | |abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6.''' | ||
The [[O2k-Fluorescence LED2-Module]] is an extension of the OROBOROS Oxygraph-2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production. | The [[O2k-Fluorescence LED2-Module]] is an extension of the OROBOROS Oxygraph-2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production. | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
:>> [[Permeabilized muscle fibres]] | :>> [[Permeabilized muscle fibres]] | ||
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|instruments=Oxygraph-2k, Fluorometry, Protocol | |instruments=Oxygraph-2k, Fluorometry, Protocol | ||
|additional=O2k-Demo, Mt-Preparation | |additional=O2k-Demo, Mt-Preparation | ||
}} | }} | ||
Revision as of 16:53, 17 May 2014
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» Open Access-MiPNet18.05.pdf; Versions
OROBOROS (2013-09) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6.
The O2k-Fluorescence LED2-Module is an extension of the OROBOROS Oxygraph-2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
- >> Permeabilized muscle fibres
• O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, Mt-Preparation
