Difference between revisions of "MiPNet18.05 Amplex-Mouse-heart"

Latest revision as of 12:30, 25 October 2023

MiPNet18.05 Amplex-Mouse-heart

O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate.

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Oroboros (2019-06-24) Mitochondr Physiol Network

Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6.

» Product: Oroboros O2k, O2k-Catalogue

» Permeabilized muscle fibers

• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom

Labels: MiParea: Respiration

Organism: Mouse Tissue;cell: Heart Preparation: Homogenate

Regulation: Cyt c Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol

O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder, H202

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+{{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRFR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate.
 |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/2/2a/MiPNet18.05_Amplex-Mouse-heart.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.05_Amplex-Mouse-heart.pdf Versions]
 |authors=Oroboros
-|year=2015-06-16
+|year=2019-06-24
 |journal=Mitochondr Physiol Network
-|abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRFR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6.'''
+|abstract=Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6.
-The [[O2k-Fluorescence LED2-Module]] is an extension of the Oroboros O2k for combining High-Resolution FluoRespirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H<sub>2</sub>O<sub>2</Sub> production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H<sub>2</sub>O<sub>2</Sub> production.
-:>> Product: [[Oroboros O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]]
+:» Product: [[Oroboros O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]]
-:>> [[Permeabilized muscle fibres]]
+:» [[Permeabilized muscle fibers]]
 |mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom
 }}
 {{Labeling
 |area=Respiration
 |organism=Mouse
 |tissues=Heart
 |preparations=Homogenate
 |topics=Cyt c
 |couplingstates=LEAK, OXPHOS, ET
 |pathways=N, S, NS
 |instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
-|additional=O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder
+|additional=O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder, H202
 }}