Difference between revisions of "MiPNet17.17 Amplex-Mouse-brain"

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O2k-Fluorometry: HRR and H₂O₂ production in mouse brain mitochondria. »Bioblast pdf«

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OROBOROS (2014-11-14) Mitochondr Physiol Network

Abstract: Fasching M, Sumbalova Z, Gnaiger E (2013) O2k-Fluorometry: HRR and H₂O₂ production in mouse brain mitochondria. Mitochondr Physiol Network 17.17.

>> Product: OROBOROS O2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Nervous system Preparation: Isolated mitochondria

Regulation: Uncoupler Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, O2k-Fluorometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

O2k-Demo, O2k-MultiSensor

@@ Line 6: / Line 6: @@
 |journal=Mitochondr Physiol Network
 |abstract=Fasching M, Sumbalova Z, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria. Mitochondr Physiol Network 17.17.
-[[High-resolution respirometry]] [1] is extended by MultiSensor techniques allowing the simultaneous measurement of oxygen consumption and one additional parameter (mitochondrial membrane potential, pH, Ca<sup>2+</sup>, or NO concentration [2-5]). Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels.
-Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
-# Quality control of preparations by respirometric performance.
-# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
-# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
-# Additional information is acquired for a limited amount of sample.
-# Oxygen dependence of parameters can be studied.
-Here we introduce the O2k-Fluorescence LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria.
 :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
 |mipnetlab=AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom
@@ Line 29: / Line 20: @@
 |additional=O2k-Demo, O2k-MultiSensor
 }}
-== Further Information ==
-'''References'''
-# [[O2k-Publications: O2k-Fluorometry]]
-# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
-# [[Aguirre 2010 Biochim Biophys Acta|
-Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]]
-# [http://www.oroboros.at/index.php?mipnetanalyzer MiPNetAnalyzer]
-# [[Hickey_2012_J_Comp_Physiol_B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.]]
-# [[Krumschnabel_2014_Methods_Mol_Biol| Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2014) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol [in press].]]
-# [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]]