MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver
| Eigentler A, Fontana-Ayoub M, Gnaiger E. Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. |
» MiPNet17.15. Open Access, PBI-Shredder HRR-Set
OROBOROS (
Abstract: A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR. • Keywords: Tissue homogenate, shredder
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments and methods"Instruments and methods" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property.
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
mt-Preparation
