Difference between revisions of "MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver"
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{{Publication | {{Publication | ||
|title=Eigentler A, Fontana-Ayoub M, Gnaiger E. | |title=Eigentler A, Fontana-Ayoub M, Gnaiger E. Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. | ||
|info=[http://oroboros.at/ | |info=[http://www.oroboros.at/?homogenate MiPNet17.15. Open Access], [[PBI-Shredder HRR-Set]] | ||
|authors=Eigentler A, Fontana-Ayoub M, Gnaiger E | |authors=Eigentler A, Fontana-Ayoub M, Gnaiger E | ||
|year=* | |year=* | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of mitochondrial yield and mitochondrial function as evaluated with HRR. | |abstract=A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR. | ||
|keywords=Tissue homogenate, shredder | |keywords=Tissue homogenate, shredder | ||
|mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom | |mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom | ||
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{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k, O2k-Manual, Protocol, MiPNet-Publication | |instruments=Oxygraph-2k, O2k-Manual, Protocol, MiPNet-Publication | ||
|organism=Mouse | |||
|tissues=Heart, Nervous system, Liver | |||
|preparations=Homogenate | |preparations=Homogenate | ||
|couplingstates=LEAK, OXPHOS, ETS | |||
|substratestates=CI, CII, CI+II | |||
|additional=mt-Preparation | |additional=mt-Preparation | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
[[Category:OroboPedia]] | [[Category:OroboPedia]] | ||
Revision as of 17:36, 23 June 2013
| Eigentler A, Fontana-Ayoub M, Gnaiger E. Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. |
» MiPNet17.15. Open Access, PBI-Shredder HRR-Set
Eigentler A, Fontana-Ayoub M, Gnaiger E (
Abstract: A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR. • Keywords: Tissue homogenate, shredder
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom
Labels:
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
mt-Preparation
