Difference between revisions of "MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver"
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{{Publication | {{Publication | ||
|title=Eigentler A, Fontana-Ayoub M, Gnaiger E (2012) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. | |title=Eigentler A, Fontana-Ayoub M, Gnaiger E (2012) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. | ||
|info=[[Media:MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver.pdf|'''Open Access-MiPNet17.15.pdf''']] | |info=[[Media:MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver.pdf|'''Open Access-MiPNet17.15.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet17.15_PBI-Shredder_Mouse-heart-brain-liver.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2012 | |year=2012 | ||
| Line 10: | Line 10: | ||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|keywords=Tissue homogenate, Shredder | |keywords=Tissue homogenate, Shredder | ||
|mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom | |mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods | |area=Respiration, Instruments;methods | ||
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|additional=mt-Preparation | |additional=mt-Preparation | ||
}} | }} | ||
== Further information== | == Further information== | ||
:>> [[PBI-Shredder HRR-Set]] | :>> [[PBI-Shredder HRR-Set]] | ||
Revision as of 08:51, 19 October 2013
| Eigentler A, Fontana-Ayoub M, Gnaiger E (2012) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. |
» Open Access-MiPNet17.15.pdf; Versions
OROBOROS (2012) Mitochondr Physiol Network
Abstract: O2k-Protocol for Tissue Homogenates for Diagnosis of Mitochondrial Respiratory Function: Mouse Heart, Brain and Liver
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• Keywords: Tissue homogenate, Shredder
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
mt-Preparation
Further information
- Version 1: 2012
- Version 3: 2013-01-15
