Difference between revisions of "MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver"
Beno Marija (talk | contribs) |
|||
Line 18: | Line 18: | ||
|tissues=Heart, Nervous system, Liver | |tissues=Heart, Nervous system, Liver | ||
|preparations=Homogenate | |preparations=Homogenate | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
|pathways=N, S, NS | |pathways=N, S, NS | ||
|instruments=Oxygraph-2k, O2k-Manual, O2k-Protocol | |instruments=Oxygraph-2k, O2k-Manual, O2k-Protocol |
Revision as of 15:19, 13 November 2017
Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. |
Oroboros (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6. Β»Versions
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
- >> O2k-Protocols: Overall contents
- >> Product: Oroboros O2k, O2k-Catalogue
β’ Keywords: Tissue homogenate, Shredder
β’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS
HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol
Mt-Preparation, PBI-Shredder