MiPNet17.03 Shredder vs Fibers
| Draxl A, Eigentler A, Gnaiger E. Mitochondrial respiration in permeabilized fibres versus homogenate from mouse
myocardium. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03. |
Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E (2012) Mitochondr Physiol Network
Abstract: In the present study we compared mitochondrial function of permeabilized fibres and homogenate of heart muscle of mice. In addition, respiration of trout heart homogenate preparations were compared with permeabilized fibres, and the PBI-Shredder was successfully tested with preparation of trout liver. • Keywords: Tissue homogenate, shredder
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E
Labels:
Preparation: Homogenate
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., O2k-Manuals & Protocols"O2k-Manuals & Protocols" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
mt-Preparation
References
- Gross VS, Greenberg HK, Baranov SV, Carlson GM, Stavrovskaya IG, Lazarev AV, Kristal BS (2011) Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization. Analyt Biochem 418: 213-223.
- Doerrier C, Draxl A, Wiethüchter A, Eigentler A, Gnaiger E (2012) Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. An application study with the PBI-Shredder. Mitocondr Physol Network 17.03: 1-12.
- Pressure BioSciences Inc. The Shredder SG3 and Shredder PULSE Tubes: Product Specification Sheet: 1-2.
- Pressure BioSciences Inc. The Shredder SG3: User Manual: 1-16.
Mitochondr Physiol Network – MiPNet Manuals and Protocols
Author contributions and progress
Prepared by Doerrier Velasco C, Draxl A, Eigentler A and Gnaiger E. DC, Wiethüchter A and DA performed the experiments.
- First on-line version: 2012-05-14.
