Difference between revisions of "MiPNet10.04 CellRespiration"

Revision as of 10:33, 29 November 2013

Garedew A, Haffner B, Huetter E, Gnaiger E (2005) An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.

» Open Access-MiPNet10.04.pdf; Versions

OROBOROS (2005) Mitochondr Physiol Network

Abstract: O2k-Protocol for Phosphorylation Control in Cell Respiration

Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations [1] of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k [2]. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis [3]. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).

>> O2k-Protocols:Overall contents

>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells

Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, TIP2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

O2k-Demo

Further information

>> MiPNet10.04

Version 6: 2012-04-07
Version 1: 2005

References

@@ Line 8: / Line 8: @@
 Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS
-Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
+Oxygraph-2k (O2k). The experiment demonstrates manual titrations [http://bioblast.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf] of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k [http://www.bioblast.at/images/8/8e/MiPNet04.05_Titration-Injection.pdf]. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis [http://www.bioblast.at/images/2/28/MiPNet12.09_O2FluxAnalysis.pdf]. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).
 :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
 :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
 |mipnetlab=AT_Innsbruck_OROBOROS
 }}
 {{Labeling
 |area=Respiration, Instruments;methods
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 |additional=O2k-Demo
 }}
 == Further information ==
 :>> [http://www.oroboros.at/?protocols_cell_hrr MiPNet10.04]