MiPNet08.15 Complex-I: Difference between revisions
No edit summary |
No edit summary |
||
Line 8: | Line 8: | ||
Complex I (CI) is the segment of the electron transport system (integral enzyme of the inner mitochondrial membrane) responsible for electron transfer from NADH to ubiquinone. CI is sensitive to different pathologies, particularly to oxidative stress, which is involved in ischemia-reperfusion injury, anoxia/ reoxygenation, aging, etc (Kuznetsov et al 2004; Rouslin & Millard 1981; Rouslin & Ranganathan, 1983; Rouslin, 1983). For the assessment of CI activity, among the ubiquinone isoprenologs, it is most convenient to use ubiquinone-1 (CoQ1) as electron acceptor, because of its relative water solubility. Importantly, CoQ1 yields one of the lowest rotenone insensitive rates and a high enzymatic rate. It is, therefore, the best electron acceptors for the CI assay. | Complex I (CI) is the segment of the electron transport system (integral enzyme of the inner mitochondrial membrane) responsible for electron transfer from NADH to ubiquinone. CI is sensitive to different pathologies, particularly to oxidative stress, which is involved in ischemia-reperfusion injury, anoxia/ reoxygenation, aging, etc (Kuznetsov et al 2004; Rouslin & Millard 1981; Rouslin & Ranganathan, 1983; Rouslin, 1983). For the assessment of CI activity, among the ubiquinone isoprenologs, it is most convenient to use ubiquinone-1 (CoQ1) as electron acceptor, because of its relative water solubility. Importantly, CoQ1 yields one of the lowest rotenone insensitive rates and a high enzymatic rate. It is, therefore, the best electron acceptors for the CI assay. | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [OROBOROS O2k-Catalogue | O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[OROBOROS O2k-Catalogue | O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
}} | }} |
Revision as of 10:01, 23 March 2016
Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochondrial membrane enzyme. |
OROBOROS (2010-08-23) Mitochondr Physiol Network
Abstract: Kuznetsov AV, Gnaiger E. Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochondrial membrane enzyme. Mitochondr Physiol Network 08.15. ยปVersions
Complex I (CI) is the segment of the electron transport system (integral enzyme of the inner mitochondrial membrane) responsible for electron transfer from NADH to ubiquinone. CI is sensitive to different pathologies, particularly to oxidative stress, which is involved in ischemia-reperfusion injury, anoxia/ reoxygenation, aging, etc (Kuznetsov et al 2004; Rouslin & Millard 1981; Rouslin & Ranganathan, 1983; Rouslin, 1983). For the assessment of CI activity, among the ubiquinone isoprenologs, it is most convenient to use ubiquinone-1 (CoQ1) as electron acceptor, because of its relative water solubility. Importantly, CoQ1 yields one of the lowest rotenone insensitive rates and a high enzymatic rate. It is, therefore, the best electron acceptors for the CI assay.
- >> Product: OROBOROS O2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Preparation: Enzyme
Enzyme: Complex I
HRR: O2k-Protocol
Mt- and marker-enzymes