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Difference between revisions of "McCurdy 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"

From Bioblast
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|year=2015
|year=2015
|event=Fatty Acid Oxidation O2k-Network Discussion Forum
|event=Fatty Acid Oxidation O2k-Network Discussion Forum
|abstract=We have had good success using the Octanoylcarnitine at the recommended dose of 0.2mM in SUIT protocols with PMG and with just GM. Respiration increases with addition of ADP and subsequent substrate additions. We have also used palmitoylcarnitine at a conc of 0.025 -0.05mM with mixed success. Often there is no or little stable increase in ADP stimulated respiration and requires addition of pyr and/or Glut. We primarily test nonhuman primate (japanses macaque) permeabilized muscle fibers. Β 
|abstract=We have had good success using the Octanoylcarnitine at the recommended dose of 0.2mM in SUIT protocols with PMG and with just GM. Respiration increases with addition of ADP and subsequent substrate additions. We have also used palmitoylcarnitine at a conc of 0.025 -0.05mM with mixed success. Often there is no or little stable increase in ADP stimulated respiration and requires addition of pyr and/or Glut. We primarily test nonhuman primate (japanses macaque) permeabilized muscle fibers.
}}
}}
{{Labeling
{{Labeling
|organism=Other mammals
|organism=Other mammals
|substratestates=FAO
|tissues=Skeletal muscle
|additional=Octanoylcarnitine + Palmitoylcarnitine Β 
|preparations=Permeabilized tissue
|couplingstates=OXPHOS
|pathways=F, N
|additional=Octanoylcarnitine + Palmitoylcarnitine
}}
}}

Latest revision as of 08:25, 8 November 2016

Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

McCurdy CE (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

We have had good success using the Octanoylcarnitine at the recommended dose of 0.2mM in SUIT protocols with PMG and with just GM. Respiration increases with addition of ADP and subsequent substrate additions. We have also used palmitoylcarnitine at a conc of 0.025 -0.05mM with mixed success. Often there is no or little stable increase in ADP stimulated respiration and requires addition of pyr and/or Glut. We primarily test nonhuman primate (japanses macaque) permeabilized muscle fibers.


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Organism: Other mammals  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: OXPHOS  Pathway: F, N 


Octanoylcarnitine + Palmitoylcarnitine