Malate: Difference between revisions
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|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|topics=Substrate; Glucose; TCA Cycle | |topics=Substrate; Glucose; TCA Cycle | ||
|instruments=Chemicals; Media | |||
|type=Respiration | |||
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== Application in [[HRR]] == | |||
'''M: Malate''' (L-Malic acid, C<sub>4</sub>H<sub>6</sub>0<sub>5</sub>; Sigma M 1000(R.T.): FW = 134.1) | |||
'''Preparation of 500 mM L-Malic acid stock solution''' (dissolved in H<sub>2</sub>O): | |||
::1) weigh 1.073 g of L-Malic acid and transfer to 10 mL volumetric glass flask | |||
::2) add 3 ml H<sub>2</sub>O | |||
::3) neutralize with 10 N KOH (approx. 750 µl) | |||
::4) check pH and adjust to 7 if necessary (add approx. another 2 µl 10 N KOH) | |||
::5) adjust final volume to 5 ml and divide into 0.2 ml portions. | |||
::6) store frozen at -20 °C | |||
Revision as of 10:40, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Malate
Description
Mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. Glutamate and malate are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations.
Abbreviation: M
Reference: MiPNet11.04; MiPNet09.12
Labels:
Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
M: Malate (L-Malic acid, C4H605; Sigma M 1000(R.T.): FW = 134.1)
Preparation of 500 mM L-Malic acid stock solution (dissolved in H2O):
- 1) weigh 1.073 g of L-Malic acid and transfer to 10 mL volumetric glass flask
- 2) add 3 ml H2O
- 3) neutralize with 10 N KOH (approx. 750 µl)
- 4) check pH and adjust to 7 if necessary (add approx. another 2 µl 10 N KOH)
- 5) adjust final volume to 5 ml and divide into 0.2 ml portions.
- 6) store frozen at -20 °C
