Malate: Difference between revisions
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'''M: Malate''' (L-Malic acid, C<sub>4</sub>H<sub>6</sub>0<sub>5</sub>); Sigma M 1000, 100 g, store at R.T.: FW = 134.1) | '''M: Malate''' (L-Malic acid, C<sub>4</sub>H<sub>6</sub>0<sub>5</sub>); Sigma M 1000, 100 g, store at R.T.: FW = 134.1) | ||
'''Preparation of | '''Preparation of 800 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) weigh 536.5 mg of L-Malic acid | ::1) weigh 536.5 mg of L-Malic acid | ||
Revision as of 10:46, 5 April 2011
- high-resolution terminology - matching measurements at high-resolution
Malate
Description
Mitochondrial respiration depends on a continuous flow of substrates across the mitochondrial membranes into the matrix space. Glutamate and malate are anions which cannot permeate through the lipid bilayer of membranes and hence require carriers, which is also true for pyruvate. Malate alone cannot support respiration of mt-preparations.
Abbreviation: M
Reference: MiPNet11.04; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
M: Malate (L-Malic acid, C4H605); Sigma M 1000, 100 g, store at R.T.: FW = 134.1)
Preparation of 800 mM stock solution (dissolved in H2O):
- 1) weigh 536.5 mg of L-Malic acid
- 2) add 3 ml H2O
- 3) neutralize with 10 N KOH (approx. 750 µl)
- 4) adjust final volume to 5 ml (in 5 mL volumetric glass flask)
- 5) divide into 0.2 ml portions
- 6) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl Hamilton syringe
- Final conc. in 2 ml O2k-chamber: 2 mM
