Mahapatra 2024 J Gerontol A Biol Sci Med Sci
| Mahapatra G, Gao Z, Bateman JR 3rd, Lockhart SN, Bergstrom J, Piloso JE, Craft S, Molina AJA (2024) Peripheral blood cells from older adults exhibit sex-associated differences in mitochondrial function. J Gerontol A Biol Sci Med Sci [Epub ahead of print]. https://doi.org/10.1093/gerona/glae098 |
Mahapatra Gargi, Gao Zhengrong, Bateman James R 3rd, Lockhart Samuel Neal, Bergstrom Jaclyn, Piloso Jemima Elizabeth, Craft Suzanne, Molina Anthony JA (2024) J Gerontol A Biol Sci Med Sci
Abstract: Blood based mitochondrial bioenergetic profiling is a feasible, economical, and minimally invasive approach that can be used to examine mitochondrial function and energy metabolism in human subjects. In this study, we use two complementary respirometric techniques to evaluate mitochondrial bioenergetics in both intact and permeabilized peripheral blood mononuclear cells (PBMCs) and platelets to examine sex dimorphism in mitochondrial function among older adults. Employing equal numbers of PBMCs and platelets to assess mitochondrial bioenergetics, we observe significantly higher respiration rates in female compared to male participants. Mitochondrial bioenergetic differences remain significant after controlling for independent parameters including demographic parameters (age, years of education), and cognitive parameters (mPACC5, COGDX). Our study illustrates that circulating blood cells, immune cells in particular, have distinctly different mitochondrial bioenergetic profiles between females and males. These differences should be taken into account as blood based bioenergetic profiling is now commonly used to understand the role of mitochondrial bioenergetics in human health and aging. • Keywords: Sex differences, Lymphocytes, Mitochondrial bioenergetics, Neutrophils, Peripheral blood mononuclear cells, Platelets • Bioblast editor: Plangger M • O2k-Network Lab: US CA San Diego Molina AJA
Labels: MiParea: Respiration
HRR: Oxygraph-2k
2024-04
