Difference between revisions of "Long 2019 Methods Mol Biol"
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{{Publication | {{Publication | ||
|title=Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros 2k | |title=Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/31041752 PMID: 31041752] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/31041752 PMID: 31041752] | ||
|authors=Long | |authors=Long Qinqiang, Huang Lizhen, Huang Kai, Yang Qinglin | ||
|year=2019 | |year=2019 | ||
|journal=Methods Mol Biol | |journal=Methods Mol Biol | ||
|abstract=In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros 2k | |abstract=In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues. | ||
|keywords=Intermyofibrillar mitochondria (IFM), Mitochondria extraction, Mitochondrial oxygen consumption, Mouse heart, Oxygraph, Subsarcolemmal mitochondria (SSM) | |keywords=Intermyofibrillar mitochondria (IFM), Mitochondria extraction, Mitochondrial oxygen consumption, Mouse heart, Oxygraph, Subsarcolemmal mitochondria (SSM) | ||
|editor=[[Plangger M]], | |editor=[[Plangger M]], | ||
|mipnetlab=CN Wuhan Huang K | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|pathways=N, S, CIV, NS, ROX | |pathways=N, S, CIV, NS, ROX | ||
|instruments=Oxygraph-2k, O2k-Protocol | |instruments=Oxygraph-2k, O2k-Protocol | ||
|additional=Labels, 2019-06, | |additional=Labels, 2019-06, CN | ||
}} | }} | ||
Latest revision as of 18:21, 29 February 2020
| Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46. |
Long Qinqiang, Huang Lizhen, Huang Kai, Yang Qinglin (2019) Methods Mol Biol
Abstract: In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues. • Keywords: Intermyofibrillar mitochondria (IFM), Mitochondria extraction, Mitochondrial oxygen consumption, Mouse heart, Oxygraph, Subsarcolemmal mitochondria (SSM) • Bioblast editor: Plangger M • O2k-Network Lab: CN Wuhan Huang K
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart
Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k, O2k-Protocol
Labels, 2019-06, CN
