Difference between revisions of "Long 2019 Methods Mol Biol"

Revision as of 14:44, 12 June 2019

Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46.

» PMID: 31041752

Long Q, Huang L, Huang K, Yang Q (2019) Methods Mol Biol

Abstract: In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues. • Keywords: Intermyofibrillar mitochondria (IFM), Mitochondria extraction, Mitochondrial oxygen consumption, Mouse heart, Oxygraph, Subsarcolemmal mitochondria (SSM) • Bioblast editor: Plangger M • O2k-Network Lab: CN Wuhan Huang K

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Heart Preparation: Isolated mitochondria

Coupling state: LEAK, OXPHOS, ET Pathway: N, S, CIV, NS, ROX HRR: Oxygraph-2k, O2k-Protocol

Labels, 2019-06

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 {{Publication
-|title=Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros 2k-Oxygraph. Methods Mol Biol 1966:237-46.
+|title=Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46.
 |info=[https://www.ncbi.nlm.nih.gov/pubmed/31041752 PMID: 31041752]
 |authors=Long Q, Huang L, Huang K, Yang Q
 |year=2019
 |journal=Methods Mol Biol
-|abstract=In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros 2k-Oxygraph system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues.
+|abstract=In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues.
 |keywords=Intermyofibrillar mitochondria (IFM), Mitochondria extraction, Mitochondrial oxygen consumption, Mouse heart, Oxygraph, Subsarcolemmal mitochondria (SSM)
 |editor=[[Plangger M]],