Difference between revisions of "Larsen 2020 Acta Physiol (Oxf)"
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{{Publication | {{Publication | ||
|title=Larsen FJ, Schiffer TA, Zinner C, Willis SJ, Morales-Alamo D, Calbet | |title=Larsen FJ, Schiffer TA, Zinner C, Willis SJ, Morales-Alamo D, Calbet J, Boushel R, Holmberg HC (2020) Mitochondrial oxygen affinity increases after sprint interval training and is related to the improvement in peak oxygen uptake. https://doi.org/10.1111/apha.13463 | ||
|info=Acta Physiol (Oxf) 229:e13463. [https://www.ncbi.nlm.nih.gov/pubmed/32144872 PMID: 32144872 Open Access] | |||
|authors=Larsen FJ, Schiffer TA, Zinner C, Willis SJ, Morales-Alamo D, Calbet | |authors=Larsen FJ, Schiffer TA, Zinner C, Willis SJ, Morales-Alamo D, Calbet JA, Boushel R, Holmberg HC | ||
|year=2020 | |year=2020 | ||
|journal=Acta Physiol (Oxf) | |journal=Acta Physiol (Oxf) | ||
|abstract=The body responds to exercise training by profound adaptations throughout the cardiorespiratory and muscular systems, which may result in improvements in maximal oxygen consumption ( | |abstract=The body responds to exercise training by profound adaptations throughout the cardiorespiratory and muscular systems, which may result in improvements in maximal oxygen consumption (VO<sub>2</sub>peak) and mitochondrial capacity. By convenience, mitochondrial respiration is often measured at supra-physiological oxygen levels, an approach that ignores any potential regulatory role of mitochondrial affinity for oxygen (p50<sub>mito</sub>) at physiological oxygen levels. | ||
In this study, we examined the | In this study, we examined the p50<sub>mito</sub> of mitochondria isolated from the ''Vastus lateralis'' and ''Triceps brachii'' in 12 healthy volunteers before and after a training intervention with 7 sessions of sprint interval training using both leg cycling and arm cranking. The changes in p50<sub>mito</sub> were compared to changes in whole-body VO<sub>2</sub>peak. | ||
We here show that | We here show that p50<sub>mito</sub> is similar in isolated mitochondria from the ''Vastus'' (40 ± 3.8 Pa) compared to ''Triceps'' (39 ± 3.3) but decreases (mitochondrial oxygen affinity increases) after 7 sessions of sprint interval training (to 26 ± 2.2 Pa in ''Vastus'' and 22 ± 2.7 Pa in ''Triceps'', both p<0.01). The change in VO<sub>2</sub>peak modeled from changes in p50<sub>mito</sub> was correlated to actual measured changes in VO<sub>2</sub>peak (R<sup>2</sup>=0.41, p=0.002). | ||
Together with mitochondrial respiratory capacity, | Together with mitochondrial respiratory capacity, p50<sub>mito</sub> is a critical factor when measuring mitochondrial function, it can decrease with sprint interval training and should be considered in the integrative analysis of the oxygen cascade from lung to mitochondria. | ||
<small>This article is protected by copyright. All rights reserved.</small> | <small>This article is protected by copyright. All rights reserved.</small> | ||
|keywords=Mitochondria, Exercise, High intensity training, Maximal oxygen consumption, Oxygen affinity, Sprint training, Training | |keywords=Mitochondria, Exercise, High intensity training, Maximal oxygen consumption, Oxygen affinity, Sprint training, Training | ||
|editor=[[Plangger M]], | |editor=[[Plangger M]], | ||
|mipnetlab=SE Stockholm Larsen FJ, SE Stockholm Weitzberg E, ES CN Las Palmas Calbet JAL, CA Vancouver Boushel RC | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Exercise physiology;nutrition;life style | ||
|organism=Human | |||
|tissues=Skeletal muscle | |||
|preparations=Isolated mitochondria | |||
|topics=Oxygen kinetics | |||
|couplingstates=OXPHOS | |||
|pathways=N, NS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2020-03, | |additional=2020-03, | ||
MitoFit2022Hypoxia | |||
}} | }} |
Latest revision as of 23:20, 15 July 2022
Larsen FJ, Schiffer TA, Zinner C, Willis SJ, Morales-Alamo D, Calbet J, Boushel R, Holmberg HC (2020) Mitochondrial oxygen affinity increases after sprint interval training and is related to the improvement in peak oxygen uptake. https://doi.org/10.1111/apha.13463 |
» Acta Physiol (Oxf) 229:e13463. PMID: 32144872 Open Access
Larsen FJ, Schiffer TA, Zinner C, Willis SJ, Morales-Alamo D, Calbet JA, Boushel R, Holmberg HC (2020) Acta Physiol (Oxf)
Abstract: The body responds to exercise training by profound adaptations throughout the cardiorespiratory and muscular systems, which may result in improvements in maximal oxygen consumption (VO2peak) and mitochondrial capacity. By convenience, mitochondrial respiration is often measured at supra-physiological oxygen levels, an approach that ignores any potential regulatory role of mitochondrial affinity for oxygen (p50mito) at physiological oxygen levels.
In this study, we examined the p50mito of mitochondria isolated from the Vastus lateralis and Triceps brachii in 12 healthy volunteers before and after a training intervention with 7 sessions of sprint interval training using both leg cycling and arm cranking. The changes in p50mito were compared to changes in whole-body VO2peak.
We here show that p50mito is similar in isolated mitochondria from the Vastus (40 ± 3.8 Pa) compared to Triceps (39 ± 3.3) but decreases (mitochondrial oxygen affinity increases) after 7 sessions of sprint interval training (to 26 ± 2.2 Pa in Vastus and 22 ± 2.7 Pa in Triceps, both p<0.01). The change in VO2peak modeled from changes in p50mito was correlated to actual measured changes in VO2peak (R2=0.41, p=0.002).
Together with mitochondrial respiratory capacity, p50mito is a critical factor when measuring mitochondrial function, it can decrease with sprint interval training and should be considered in the integrative analysis of the oxygen cascade from lung to mitochondria.
This article is protected by copyright. All rights reserved. • Keywords: Mitochondria, Exercise, High intensity training, Maximal oxygen consumption, Oxygen affinity, Sprint training, Training • Bioblast editor: Plangger M • O2k-Network Lab: SE Stockholm Larsen FJ, SE Stockholm Weitzberg E, ES CN Las Palmas Calbet JAL, CA Vancouver Boushel RC
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Regulation: Oxygen kinetics Coupling state: OXPHOS Pathway: N, NS HRR: Oxygraph-2k
2020-03, MitoFit2022Hypoxia